Cited 13 times in
The potential effects of ethyl acetate fraction from Curcuma longa L. on lipolysis in differentiated 3T3-L1 adipocytes
DC Field | Value | Language |
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dc.contributor.author | 윤호근 | - |
dc.date.accessioned | 2015-04-23T17:31:33Z | - |
dc.date.available | 2015-04-23T17:31:33Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1096-620X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/102634 | - |
dc.description.abstract | The effects of the turmeric ethyl acetate fraction (TEF) from the methanolic extract from Curcuma longa L. on lipid metabolism and underlying mechanisms of lipolysis were investigated in 3T3-L1 adipocytes. The intracellular lipid droplets were stained with Oil red O dye and quantified. Compared to the control, lipid accumulation was significantly decreased by 46.6% with treatment by TEF at the concentration of 20 microg/mL. The intracellular triglyceride (TG) level was also reduced by 37.9% at the concentration of 20 microg/mL. To determine the mechanism for TG content reduction, levels of glucose uptake and glycerol release were measured. Incubation of the 3T3-L1 adipocytes with TEF for 4 hours significantly lowered the cellular level of glucose in a dose-dependent manner. Furthermore, cellular expression of insulin-responsive glucose transporter (GLUT)-4 was decreased by 46%, indicating that reduced glucose uptake was due to a decrease in cellular GLUT-4 expression. In addition, the level of free glycerol released into the cultured medium was increased by 36.4% with the treatment by TEF. In subsequent measurements using quantitative real-time polymerase chain reaction, mRNA levels of hormone-sensitive lipase (HSL) and adipose TG lipase (ATGL) were elevated by 34.8% and 16.9%, respectively, at the concentration of 20 microg/mL. These results suggest that TEF partially inhibits lipogenesis by the suppression of glucose uptake via the decreased expression of cellular GLUT-4 and stimulates lipolysis through the induction of HSL and/or ATGL gene expression, resulting in the increased glycerol release. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 364~370 | - |
dc.relation.isPartOf | JOURNAL OF MEDICINAL FOOD | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | 3T3-L1 Cells | - |
dc.subject.MESH | Adipocytes/drug effects* | - |
dc.subject.MESH | Adipocytes/metabolism | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Biological Transport | - |
dc.subject.MESH | Curcuma/chemistry* | - |
dc.subject.MESH | Dose-Response Relationship, Drug | - |
dc.subject.MESH | Glucose/metabolism* | - |
dc.subject.MESH | Glucose Transporter Type 4/metabolism* | - |
dc.subject.MESH | Glycerol/metabolism | - |
dc.subject.MESH | Lipase/genetics | - |
dc.subject.MESH | Lipase/metabolism | - |
dc.subject.MESH | Lipolysis/drug effects* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Organelles/drug effects | - |
dc.subject.MESH | Organelles/metabolism | - |
dc.subject.MESH | Plant Extracts/pharmacology* | - |
dc.subject.MESH | RNA, Messenger/metabolism | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Sterol Esterase/genetics | - |
dc.subject.MESH | Sterol Esterase/metabolism | - |
dc.subject.MESH | Triglycerides/metabolism | - |
dc.title | The potential effects of ethyl acetate fraction from Curcuma longa L. on lipolysis in differentiated 3T3-L1 adipocytes | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Biochemistry & Molecular Biology (생화학,분자생물학) | - |
dc.contributor.googleauthor | Jeongmin Lee | - |
dc.contributor.googleauthor | Ho-Geun Yoon | - |
dc.contributor.googleauthor | Yoo-Hyun Lee | - |
dc.contributor.googleauthor | Jeongjin Park | - |
dc.contributor.googleauthor | Yanghee You | - |
dc.contributor.googleauthor | Kyungmi Kim | - |
dc.contributor.googleauthor | Ja-Young Jang | - |
dc.contributor.googleauthor | Ji-Won Yang | - |
dc.contributor.googleauthor | Woojin Jun | - |
dc.identifier.doi | 10.1089/jmf.2009.1276 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02625 | - |
dc.relation.journalcode | J01589 | - |
dc.identifier.eissn | 1557-7600 | - |
dc.identifier.pmid | 20412020 | - |
dc.identifier.url | http://online.liebertpub.com/doi/abs/10.1089/jmf.2009.1276 | - |
dc.contributor.alternativeName | Yoon, Ho Geun | - |
dc.contributor.affiliatedAuthor | Yoon, Ho Geun | - |
dc.citation.volume | 13 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 364 | - |
dc.citation.endPage | 370 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MEDICINAL FOOD, Vol.13(2) : 364-370, 2010 | - |
dc.identifier.rimsid | 55340 | - |
dc.type.rims | ART | - |
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