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Robust enhancement of neural differentiation from human ES and iPS cells regardless of their innate difference in differentiation propensity

Authors
 Dae-Sung Kim  ;  Jae Souk Lee  ;  Joong Woo Leem  ;  Yong Jun Huh  ;  Ji Young Kim  ;  Han-Soo Kim  ;  In-Hyun Park  ;  George Q. Daley  ;  Dong-Youn Hwang  ;  Dong-Wook Kim 
Citation
 STEM CELL REVIEWS AND REPORTS, Vol.6(2) : 270-281, 2010 
Journal Title
 STEM CELL REVIEWS AND REPORTS 
ISSN
 1550-8943 
Issue Date
2010
MeSH
Blotting, Western ; Cell Differentiation/physiology* ; Cell Line ; Embryonic Stem Cells/cytology* ; Humans ; Induced Pluripotent Stem Cells/cytology* ; Neurons/cytology* ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction/physiology
Keywords
Pluripotent stem cell  ; Differentiation propensity  ; Neural induction  ; Cell signaling  ; Small molecule 
Abstract
Our analyses of three human induced pluripotent stem cell (hiPSC) and six human embryonic stem cell (hESC) lines showed marked variability in differentiation potential into specific lineages, which often hampers their differentiation into specific cell types or cell lineages of interest. Simultaneous inhibition of both Activin/Nodal and BMP pathways with small molecules, SB431542 and dorsomorphin (DM), respectively, promoted significant neural differentiation from all human pluripotent stem cell (hPSC) lines tested, regardless of their differentiation propensity. On the contrary, differentiation into other cell lineages and the number of undifferentiated cells were significantly reduced after differentiation by the dual inhibition. These results demonstrate that innate differentiation propensity of hPSCs could be overcome, at least in part, by modulation of intracellular signaling pathways, resulting in efficient generation of desirable cell types, such as neural cells.
Full Text
http://link.springer.com/article/10.1007%2Fs12015-010-9138-1
DOI
10.1007/s12015-010-9138-1
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dae Sung(김대성)
Kim, Dong Wook(김동욱) ORCID logo https://orcid.org/0000-0002-5025-1532
Kim, Ji Young(김지영)
Kim, Han Soo(김한수)
Leem, Joong Woo(임중우) ORCID logo https://orcid.org/0000-0002-1605-2230
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101813
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