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Robust enhancement of neural differentiation from human ES and iPS cells regardless of their innate difference in differentiation propensity

DC FieldValueLanguage
dc.contributor.author김대성-
dc.contributor.author김동욱-
dc.contributor.author김지영-
dc.contributor.author김한수-
dc.contributor.author임중우-
dc.date.accessioned2015-04-23T17:05:48Z-
dc.date.available2015-04-23T17:05:48Z-
dc.date.issued2010-
dc.identifier.issn1550-8943-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/101813-
dc.description.abstractOur analyses of three human induced pluripotent stem cell (hiPSC) and six human embryonic stem cell (hESC) lines showed marked variability in differentiation potential into specific lineages, which often hampers their differentiation into specific cell types or cell lineages of interest. Simultaneous inhibition of both Activin/Nodal and BMP pathways with small molecules, SB431542 and dorsomorphin (DM), respectively, promoted significant neural differentiation from all human pluripotent stem cell (hPSC) lines tested, regardless of their differentiation propensity. On the contrary, differentiation into other cell lineages and the number of undifferentiated cells were significantly reduced after differentiation by the dual inhibition. These results demonstrate that innate differentiation propensity of hPSCs could be overcome, at least in part, by modulation of intracellular signaling pathways, resulting in efficient generation of desirable cell types, such as neural cells.-
dc.description.statementOfResponsibilityopen-
dc.format.extent270~281-
dc.relation.isPartOfSTEM CELL REVIEWS AND REPORTS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCell Differentiation/physiology*-
dc.subject.MESHCell Line-
dc.subject.MESHEmbryonic Stem Cells/cytology*-
dc.subject.MESHHumans-
dc.subject.MESHInduced Pluripotent Stem Cells/cytology*-
dc.subject.MESHNeurons/cytology*-
dc.subject.MESHReverse Transcriptase Polymerase Chain Reaction-
dc.subject.MESHSignal Transduction/physiology-
dc.titleRobust enhancement of neural differentiation from human ES and iPS cells regardless of their innate difference in differentiation propensity-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Laboratory Medicine (진단검사의학)-
dc.contributor.googleauthorDae-Sung Kim-
dc.contributor.googleauthorJae Souk Lee-
dc.contributor.googleauthorJoong Woo Leem-
dc.contributor.googleauthorYong Jun Huh-
dc.contributor.googleauthorJi Young Kim-
dc.contributor.googleauthorHan-Soo Kim-
dc.contributor.googleauthorIn-Hyun Park-
dc.contributor.googleauthorGeorge Q. Daley-
dc.contributor.googleauthorDong-Youn Hwang-
dc.contributor.googleauthorDong-Wook Kim-
dc.identifier.doi10.1007/s12015-010-9138-1-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00367-
dc.contributor.localIdA01100-
dc.contributor.localIdA03409-
dc.contributor.localIdA00981-
dc.contributor.localIdA00406-
dc.relation.journalcodeJ02682-
dc.identifier.eissn1558-6804-
dc.identifier.pmid20376579-
dc.identifier.urlhttp://link.springer.com/article/10.1007%2Fs12015-010-9138-1-
dc.subject.keywordPluripotent stem cell -
dc.subject.keywordDifferentiation propensity -
dc.subject.keywordNeural induction -
dc.subject.keywordCell signaling -
dc.subject.keywordSmall molecule -
dc.contributor.alternativeNameKim, Dae Sung-
dc.contributor.alternativeNameKim, Dong Wook-
dc.contributor.alternativeNameKim, Ji Young-
dc.contributor.alternativeNameKim, Han Soo-
dc.contributor.alternativeNameLeem, Joong Woo-
dc.contributor.affiliatedAuthorKim, Dae Sung-
dc.contributor.affiliatedAuthorKim, Han Soo-
dc.contributor.affiliatedAuthorLeem, Joong Woo-
dc.contributor.affiliatedAuthorKim, Ji Young-
dc.contributor.affiliatedAuthorKim, Dong Wook-
dc.citation.volume6-
dc.citation.number2-
dc.citation.startPage270-
dc.citation.endPage281-
dc.identifier.bibliographicCitationSTEM CELL REVIEWS AND REPORTS, Vol.6(2) : 270-281, 2010-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers

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