1 235

Cited 27 times in

The effects of COX-2 inhibitor during osteogenic differentiation of bone marrow-derived human mesenchymal stem cells.

 Dong Suk Yoon  ;  Je Hyun Yoo  ;  Yun Hee Kim  ;  Seungil Paik  ;  Chang Dong Han  ;  Jin Woo Lee 
 STEM CELLS AND DEVELOPMENT, Vol.19(10) : 1523-1533, 2010 
Journal Title
Issue Date
Adult ; Aged ; Anti-Inflammatory Agents, Non-Steroidal/pharmacology ; Anti-Inflammatory Agents, Non-Steroidal/therapeutic use ; Biomarkers/metabolism ; Bone Marrow Cells/cytology ; Bone Marrow Cells/physiology* ; Celecoxib ; Cell Differentiation/drug effects* ; Cyclooxygenase 2/metabolism* ; Cyclooxygenase 2 Inhibitors/pharmacology* ; Female ; Gene Expression Regulation/drug effects ; Humans ; Inflammation/chemically induced ; Inflammation/drug therapy ; Inflammation/genetics ; Interleukin-1beta/pharmacology ; Male ; Mesenchymal Stromal Cells/cytology ; Mesenchymal Stromal Cells/drug effects* ; Mesenchymal Stromal Cells/physiology* ; Middle Aged ; Naproxen/pharmacology ; Osteogenesis/drug effects* ; Pyrazoles/pharmacology ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Sulfonamides/pharmacology ; Young Adult
Cyclooxygenase-2 (COX-2) inhibitors suppress bone repair and bone formation by suppressing angiogenesis as well as potentially interfering with osteoblast and osteoclast functions. In spite of these reports, there is a controversy over the exact effects of COX-2 inhibitors on bone formation processes itself. This work was designed to investigate the effect of COX-2 inhibitor on osteogenesis of human bone marrow-derived mesenchymal stem cells (MSC). MSCs in osteogenesis were treated with COX-2 inhibitor (celecoxib and naproxen) in the absence or presence of interleukin-1β (IL-1β), which was used to induce inflammation. Following differentiation, alkaline phosphatase (ALP) and calcium contents of IL-1β-treated MSC were significantly reduced by high doses of COX-2 inhibitors compared with the low-dose group. However, in non-inflammatory-conditioned MSCs, ALP and calcium contents were not reduced by COX-2 inhibitors. The mRNA expression of Runx2/Cbf alpha 1, Dlx5, and osteocalcin was also decreased by COX-2 inhibitors in inflammatory-conditioned MSCs and showed a significant decrease for the high dose while they remained constant in the non-inflammatory-conditioned MSCs. These data indicate that the osteogenic potential of MSC is inhibited/delayed by the treatment of high-dose NSAIDs under inflammatory conditions.
Full Text
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
Yonsei Authors
Kim, Yun Hee(김윤희)
Paik, Seung Il(백승일)
Yoo, Je Hyun(유제현)
Yoon, Dong Suk(윤동석)
Lee, Jin Woo(이진우) ORCID logo https://orcid.org/0000-0002-0293-9017
Han, Chang Dong(한창동)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.