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Mapping of BrdU label-retaining dental pulp cells in growing teeth and their regenerative capacity after injuries

Authors
 Yuko Ishikawa  ;  Hiroko Ida-Yonemochi  ;  Hironobu Suzuki  ;  Kuniko Nakakura-Ohshima  ;  Han-Sung Jung  ;  Masaki J. Honda  ;  Yumiko Ishii  ;  Nobukazu Watanabe  ;  Hayato Ohshima 
Citation
 HISTOCHEMISTRY AND CELL BIOLOGY, Vol.134(3) : 227-241, 2010 
Journal Title
HISTOCHEMISTRY AND CELL BIOLOGY
ISSN
 0948-6143 
Issue Date
2010
MeSH
Adult Stem Cells/physiology* ; Animals ; Bromodeoxyuridine* ; Cell Differentiation ; Cell Proliferation ; Dental Cavity Preparation ; Dental Pulp/cytology* ; Dental Pulp/physiology ; Female ; Mesenchymal Stromal Cells/cytology* ; Pregnancy ; Rats ; Rats, Wistar ; Regeneration ; Side-Population Cells/cytology ; Tooth Injuries/physiopathology ; Tooth Replantation
Keywords
Dental cavity preparation ; Tooth replantation ; Cell proliferation ; Cell differentiation ; Rats (Wistar)
Abstract
Recent studies have demonstrated that human dental pulp contains adult stem cells. A pulse of the thymidine analog BrdU given to young animals at the optimal time could clarify where slow-cycling long-term label-retaining cells (LRCs), putative adult stem cells, reside in the pulp tissue. This study focuses on the mapping of LRCs in growing teeth and their regenerative capacity after tooth injuries. Two to seven peritoneal injections of BrdU into pregnant Wistar rats revealed slow-cycling long-term dense LRCs in the mature tissues of born animals. Numerous dense LRCs were postnatally decreased in number and reached a plateau at 4 weeks after birth when they mainly resided in the center of the dental pulp, associating with blood vessels. Mature dental pulp cells were stained with Hoechst 33342 and sorted into (<0.76%) side population cells using FACS, which included dense LRCs. Some dense LRCs co-expressed mesenchymal stem cell markers such as STRO-1 or CD146. Tooth injuries caused degeneration of the odontoblast layer, and newly differentiated odontoblast-like cells contained LRCs. Thus, dense LRCs in mature pulp tissues were supposed to be dental pulp stem cells possessing regenerative capacity for forming newly differentiated odontoblast-like cells. The present study proposes the new hypothesis that both granular and dense LRCs are equipped in the dental pulp and that the dense LRCs with proliferative capacity play crucial roles in the pulpal healing process following exogenous stimuli in cooperation with the granular LRCs.
Full Text
http://link.springer.com/article/10.1007%2Fs00418-010-0727-5
DOI
10.1007/s00418-010-0727-5
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101619
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