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Identification of adenovirus, influenza virus, parainfluenza virus, and respiratory syncytial virus by two kinds of multiplex polymerase chain reaction (PCR) and a shell vial culture in pediatric patients with viral pneumonia

 Jong-Han Lee  ;  Jin-Kyong Chun  ;  Dong Soo Kim  ;  Yongjung Park  ;  Jong Rak Choi  ;  Hyon-Suk Kim 
 YONSEI MEDICAL JOURNAL, Vol.51(5) : 761-767, 2010 
Journal Title
Issue Date
Adenoviridae/genetics ; Adenoviridae/isolation & purification* ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Infant, Newborn ; Male ; Orthomyxoviridae/genetics ; Orthomyxoviridae/isolation & purification* ; Pneumonia, Viral/virology* ; Polymerase Chain Reaction/methods* ; Respiratory Syncytial Viruses/genetics ; Respiratory Syncytial Viruses/isolation & purification* ; Respirovirus/genetics ; Respirovirus/isolation & purification*
Multiplex PCR ; respiratory virus ; virus culture ; viral pneumonia
PURPOSE: Early identification of causative agents in lower respiratory infection of pediatric patients can reduce morbidity and prevent an overuse of antimicrobials. Two kinds of multiplex polymerase chain reaction (PCR) and a commercial shell vial viral culture were performed to identify causative agents in pediatric patients. MATERIALS AND METHODS: Nasopharyngeal aspirates of 220 children diagnosed with viral pneumonia were obtained. Two kinds of multiplex PCR (Seeplex(TM) RV detection kit, and Labopass(TM) RV detection kit), and a shell vial culture by R-Mix were performed. RESULTS: Positive samples from 220 total samples by two multiplex PCRs were 52.7% and 46.4%, respectively. We also cultured 103 samples that showed positive results of the adenovirus, influenza virus, parainfluenza virus, and respiratory syncytial virus (RSV) by two multiplex PCR. The RSV was most frequently detected in 53.0% (Seeplex) and 51.7% (Labopass) of patients. The detection rate of adenovirus (AdV) was 10.3% and 12.1%, influenza virus (IFV) A and B was 12.5% and 3.4%, and parainfluenza virus (PIFV) 1, 2, and 3 were 2.9% and 2.6%. Shell vial cultures showed concordant results with each multiplex PCR by 96.1% and 77.7%, respectively. Sequencing results were 90% consistent with multiplex PCR. CONCLUSION: Multiplex PCR showed more positivity than the shell vial culture and it can be an effective primary test. Other complementary efforts such as viral cultures and sequencing analysis could be considered, according to clinical and laboratory conditions.
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1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Dong Soo(김동수)
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
Park, Yong Jung(박용정)
Lee, Jong Han(이종한)
Choi, Jong Rak(최종락) ORCID logo https://orcid.org/0000-0002-0608-2989
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