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Markers of squamous cell carcinoma in sarco/endoplasmic reticulum Ca2+ ATPase 2 heterozygote mice keratinocytes

Authors
 Jeong Hee Hong  ;  Yu-Mi Yang  ;  Hyun Sil Kim  ;  Syng-Ill Lee  ;  Shmuel Muallem  ;  Dong Min Shin 
Citation
 PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, Vol.103(1) : 81-87, 2010 
Journal Title
 PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY 
ISSN
 0079-6107 
Issue Date
2010
MeSH
Animals ; Biomarkers ; Calcium Signaling/physiology ; Carcinoma, Squamous Cell/genetics ; Carcinoma, Squamous Cell/metabolism* ; Heterozygote* ; Humans ; Keratinocytes/metabolism* ; Mice ; Sarcoplasmic Reticulum Calcium-Transporting ATPases/genetics* ; Sarcoplasmic Reticulum Calcium-Transporting ATPases/metabolism*
Keywords
Ca2þ signaling ; SERCA2þ/ ; Squamous cell carcinoma ; Keratinocytes
Abstract
A mutation of Atp2a2 gene encoding the sarco/endoplasmic reticulum Ca(2+)-ATPase 2 (SERCA2) causes Darier's disease in human and null mutation in one copy of Atp2a2 leads to a high incidence of squamous cell tumor in a mouse model. In SERCA2 heterozygote (SERCA2(+/-)) mice keratinocytes, mechanisms involved in partial depletion of SERCA2 gene and its related tumor induction have not been studied. In this study, we investigated Ca(2+) signaling and differential gene expression in primary cultured keratinocytes from SERCA2(+/-) mice. SERCA2(+/-) keratinocytes showed reduced initial increases in intracellular concentration of calcium in response to ATP, a G-protein coupled receptor agonist, and higher store-operated Ca(2+) entry with the treatment of thapsigargin, an inhibitor of SERCA, compared to wild type kerationcytes. Protein expressions of plasma membrane Ca(2+) ATPases, NFATc1, phosphorylated ERK, JNK, and phospholipase gamma1 were increased in SERCA2(+/-) keratinocytes. Using the gene fishing system, we first found in SERCA2(+/-) keratinocytes that gene level of tumor-associated calcium signal transducer 1, crystalline alphaB, procollagen XVIII alpha1, and nuclear factor I-B were increased. Expression of involucrin, a marker of keratinocyte differentiation, was decreased in SERCA2(+/-) keratinocytes. These results suggest that the alterations of Ca(2+) signaling by SERCA2 haploinsufficiency alternate the gene expression of tumor induction and differentiation in keratinocytes
Full Text
http://www.sciencedirect.com/science/article/pii/S0079610709000698
DOI
10.1016/j.pbiomolbio.2009.10.005
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Hyun Sil(김현실) ORCID logo https://orcid.org/0000-0003-3614-1764
Shin, Dong Min(신동민) ORCID logo https://orcid.org/0000-0001-6042-0435
Lee, Syng Ill(이승일)
Hong, Jeong Hee(홍정희)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101389
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