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Markers of squamous cell carcinoma in sarco/endoplasmic reticulum Ca2+ ATPase 2 heterozygote mice keratinocytes
DC Field | Value | Language |
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dc.contributor.author | 김현실 | - |
dc.contributor.author | 신동민 | - |
dc.contributor.author | 이승일 | - |
dc.contributor.author | 홍정희 | - |
dc.date.accessioned | 2015-04-23T16:52:24Z | - |
dc.date.available | 2015-04-23T16:52:24Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0079-6107 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/101389 | - |
dc.description.abstract | A mutation of Atp2a2 gene encoding the sarco/endoplasmic reticulum Ca(2+)-ATPase 2 (SERCA2) causes Darier's disease in human and null mutation in one copy of Atp2a2 leads to a high incidence of squamous cell tumor in a mouse model. In SERCA2 heterozygote (SERCA2(+/-)) mice keratinocytes, mechanisms involved in partial depletion of SERCA2 gene and its related tumor induction have not been studied. In this study, we investigated Ca(2+) signaling and differential gene expression in primary cultured keratinocytes from SERCA2(+/-) mice. SERCA2(+/-) keratinocytes showed reduced initial increases in intracellular concentration of calcium in response to ATP, a G-protein coupled receptor agonist, and higher store-operated Ca(2+) entry with the treatment of thapsigargin, an inhibitor of SERCA, compared to wild type kerationcytes. Protein expressions of plasma membrane Ca(2+) ATPases, NFATc1, phosphorylated ERK, JNK, and phospholipase gamma1 were increased in SERCA2(+/-) keratinocytes. Using the gene fishing system, we first found in SERCA2(+/-) keratinocytes that gene level of tumor-associated calcium signal transducer 1, crystalline alphaB, procollagen XVIII alpha1, and nuclear factor I-B were increased. Expression of involucrin, a marker of keratinocyte differentiation, was decreased in SERCA2(+/-) keratinocytes. These results suggest that the alterations of Ca(2+) signaling by SERCA2 haploinsufficiency alternate the gene expression of tumor induction and differentiation in keratinocytes | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 81~87 | - |
dc.relation.isPartOf | PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Biomarkers | - |
dc.subject.MESH | Calcium Signaling/physiology | - |
dc.subject.MESH | Carcinoma, Squamous Cell/genetics | - |
dc.subject.MESH | Carcinoma, Squamous Cell/metabolism* | - |
dc.subject.MESH | Heterozygote* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Keratinocytes/metabolism* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Sarcoplasmic Reticulum Calcium-Transporting ATPases/genetics* | - |
dc.subject.MESH | Sarcoplasmic Reticulum Calcium-Transporting ATPases/metabolism* | - |
dc.title | Markers of squamous cell carcinoma in sarco/endoplasmic reticulum Ca2+ ATPase 2 heterozygote mice keratinocytes | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | Jeong Hee Hong | - |
dc.contributor.googleauthor | Yu-Mi Yang | - |
dc.contributor.googleauthor | Hyun Sil Kim | - |
dc.contributor.googleauthor | Syng-Ill Lee | - |
dc.contributor.googleauthor | Shmuel Muallem | - |
dc.contributor.googleauthor | Dong Min Shin | - |
dc.identifier.doi | 10.1016/j.pbiomolbio.2009.10.005 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01121 | - |
dc.contributor.localId | A02091 | - |
dc.contributor.localId | A02924 | - |
dc.contributor.localId | A04434 | - |
dc.relation.journalcode | J02552 | - |
dc.identifier.eissn | 1873-1732 | - |
dc.identifier.pmid | 19840814 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0079610709000698 | - |
dc.subject.keyword | Ca2þ signaling | - |
dc.subject.keyword | SERCA2þ/ | - |
dc.subject.keyword | Squamous cell carcinoma | - |
dc.subject.keyword | Keratinocytes | - |
dc.contributor.alternativeName | Kim, Hyun Sil | - |
dc.contributor.alternativeName | Shin, Dong Min | - |
dc.contributor.alternativeName | Lee, Syng Ill | - |
dc.contributor.alternativeName | Hong, Jeong Hee | - |
dc.contributor.affiliatedAuthor | Kim, Hyun Sil | - |
dc.contributor.affiliatedAuthor | Shin, Dong Min | - |
dc.contributor.affiliatedAuthor | Lee, Syng Ill | - |
dc.contributor.affiliatedAuthor | Hong, Jeong Hee | - |
dc.citation.volume | 103 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 81 | - |
dc.citation.endPage | 87 | - |
dc.identifier.bibliographicCitation | PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, Vol.103(1) : 81-87, 2010 | - |
dc.identifier.rimsid | 51020 | - |
dc.type.rims | ART | - |
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