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Panel of candidate biomarkers for renal cell carcinoma

Authors
 Dong Su Kim  ;  Yoon Pyo Choi  ;  Suki Kang  ;  Ming Qing Gao  ;  Baekil Kim  ;  Haeng Ran Park  ;  Young Deuk Choi  ;  Jong Baek Lim  ;  Hyung Jin Na  ;  Hye Kyung Kim  ;  Young-Pyo Nam  ;  Mi Hyang Moon  ;  Hae Ree Yun  ;  Dong Hee Lee  ;  Won-Man Park  ;  Nam Hoon Cho 
Citation
 Journal of Proteome Research, Vol.9(7) : 3710-3719, 2010 
Journal Title
 Journal of Proteome Research 
ISSN
 1535-3893 
Issue Date
2010
Abstract
The timely diagnosis and therapeutic monitoring of human renal cell carcinoma (RCC) is limited by the lack of specific biomarkers. To identify candidate RCC biomarkers, we used 2-DE gel electrophoresis with mass spectrometry and 2-DE spot intensity-based ROC analysis to analyze 18 sets of paired normal and RCC tumor tissue including conventional, papillary, and chromophobe subtypes. Validation was performed with RCC patient plasma samples and confirmed by clustergram, shRNA, and immunohistochemistry assays. Cardinal candidates were evaluated by ELISA. The leading candidate biomarker that was upregulated in RCC samples according to the clustergram and validation analysis was nicotinamide N-methyltransferase (NNMT) (13/15, P < 0.0001). Other upregulated candidate biomarkers that were identified by this method include ferritin, hNSE, NM23, secretagogin, and L-plastin. The upregulation of NNMT in RCC was confirmed by immunoblotting and immunohistochemistry. Analysis of fractionated membrane-associated proteins identified CAP-G, mitofillin, tubulin alpha, RBBP7, and HSP27. Of these, RBBP7 and HSP27 were highly expressed in the chromophobe subtype of RCC (3/3) but were absent from conventional RCC (0/3). The triple combination of the NNMT, FTL, and hNSE biomarkers had the highest predictive capacity of 0.993, while NNMT was the single, most powerful candidate diagnostic biomarker for all types of RCC.
Full Text
http://pubs.acs.org/doi/abs/10.1021/pr100236r
DOI
10.1021/pr100236r
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Urology (비뇨의학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실)
Yonsei Authors
강숙희(Kang, Suki) ORCID logo https://orcid.org/0000-0002-9957-3479
고명청(Gao, Ming Qing)
김백길(Kim, Baek Gil) ORCID logo https://orcid.org/0000-0001-6270-1433
박행란(Park, Haeng Ran)
임종백(Lim, Jong Baeck)
조남훈(Cho, Nam Hoon) ORCID logo https://orcid.org/0000-0002-0045-6441
최영득(Choi, Young Deuk) ORCID logo https://orcid.org/0000-0002-8545-5797
최윤표(Choi, Yoon Pyo)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101368
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