New cfiA variant and novel insertion sequence elements in carbapenem-resistant Bacteroides fragilis isolates from Korea.

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Authors: Kyoung Ho Roh ; Sinyoung Kim ; Chang-Ki Kim ; Jong Hwa Yum ; Myung Sook Kim ; Dongeun Yong ; Seok Hoon Jeong ; Kyungwon Lee ; June Myung Kim ; Yunsop Chong

Citation: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, Vol.66(4) : 343-348, 2010

MeSH: Amino Acid Sequence ; Amino Acid Substitution/genetics ; Anti-Bacterial Agents/pharmacology* ; Bacterial Proteins/biosynthesis ; Bacterial Proteins/genetics* ; Bacteroides Infections/microbiology ; Bacteroides fragilis/drug effects* ; Bacteroides fragilis/genetics* ; Bacteroides fragilis/isolation & purification ; Carbapenems/pharmacology* ; DNA Transposable Elements* ; DNA, Bacterial/chemistry ; DNA, Bacterial/genetics ; Humans ; Molecular Sequence Data ; Republic of Korea ; Sequence Alignment ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; beta-Lactam Resistance* ; beta-Lactamases/biosynthesis ; beta-Lactamases/genetics*

Abstract: Of 276 nonduplicate Bacteroides fragilis clinical isolates recovered from 1997 to 2004, 3 were resistant to carbapenem. cepA and cfiA alleles were detected by polymerase chain reaction in 240 (87.0%) and 11 (4.0%) of the isolates, respectively. Insertion sequence (IS) elements were found only in the 3 carbapenem-resistant B. fragilis isolates, which produced metallo-beta-lactamase at a level detectable by UV spectrophotometry. Sequence analysis showed 1 new cfiA variant, cfiA(11), and 2 novel IS elements. The cfiA(11) gene revealed 5 amino acid substitutions compared to cfiA, with 97.6% amino acid identity. The transposase, terminal inverted repeat sequence, and target site duplication sequence of the 2 novel IS elements were unique. This study reconfirmed the correlation between ISs and carbapenem resistance in B. fragilis.

Full Text: http://www.sciencedirect.com/science/article/pii/S0732889309004490

Appears in Collections:: 1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

Yonsei Authors: Kim, Sin Young(김신영) https://orcid.org/0000-0002-2609-8945
Kim, June Myung(김준명)
Yong, Dong Eun(용동은) https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) https://orcid.org/0000-0003-3788-2134
Jeong, Seok Hoon(정석훈) https://orcid.org/0000-0001-9290-897X
Chong, Yun Sop(정윤섭)

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