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New cfiA variant and novel insertion sequence elements in carbapenem-resistant Bacteroides fragilis isolates from Korea.

Authors
 Kyoung Ho Roh  ;  Sinyoung Kim  ;  Chang-Ki Kim  ;  Jong Hwa Yum  ;  Myung Sook Kim  ;  Dongeun Yong  ;  Seok Hoon Jeong  ;  Kyungwon Lee  ;  June Myung Kim  ;  Yunsop Chong 
Citation
 DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, Vol.66(4) : 343-348, 2010 
Journal Title
 DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE 
ISSN
 0732-8893 
Issue Date
2010
MeSH
Amino Acid Sequence ; Amino Acid Substitution/genetics ; Anti-Bacterial Agents/pharmacology* ; Bacterial Proteins/biosynthesis ; Bacterial Proteins/genetics* ; Bacteroides Infections/microbiology ; Bacteroides fragilis/drug effects* ; Bacteroides fragilis/genetics* ; Bacteroides fragilis/isolation & purification ; Carbapenems/pharmacology* ; DNA Transposable Elements* ; DNA, Bacterial/chemistry ; DNA, Bacterial/genetics ; Humans ; Molecular Sequence Data ; Republic of Korea ; Sequence Alignment ; Sequence Analysis, DNA ; Sequence Homology, Amino Acid ; beta-Lactam Resistance* ; beta-Lactamases/biosynthesis ; beta-Lactamases/genetics*
Abstract
Of 276 nonduplicate Bacteroides fragilis clinical isolates recovered from 1997 to 2004, 3 were resistant to carbapenem. cepA and cfiA alleles were detected by polymerase chain reaction in 240 (87.0%) and 11 (4.0%) of the isolates, respectively. Insertion sequence (IS) elements were found only in the 3 carbapenem-resistant B. fragilis isolates, which produced metallo-beta-lactamase at a level detectable by UV spectrophotometry. Sequence analysis showed 1 new cfiA variant, cfiA(11), and 2 novel IS elements. The cfiA(11) gene revealed 5 amino acid substitutions compared to cfiA, with 97.6% amino acid identity. The transposase, terminal inverted repeat sequence, and target site duplication sequence of the 2 novel IS elements were unique. This study reconfirmed the correlation between ISs and carbapenem resistance in B. fragilis.
Full Text
http://www.sciencedirect.com/science/article/pii/S0732889309004490
DOI
10.1016/j.diagmicrobio.2009.11.003
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sin Young(김신영) ORCID logo https://orcid.org/0000-0002-2609-8945
Kim, June Myung(김준명)
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
Chong, Yun Sop(정윤섭)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101191
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