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Pleurospermum kamtschaticum extract induces apoptosis via mitochondrial pathway and NAG-1 expression in colon cancer cells.

Authors
 Jin-Eun Kim  ;  Won-Yoon Chung  ;  Won-Bae Kim  ;  Hee-Juhn Park  ;  Chang Ki Lee  ;  Kyung-Soo Chun 
Citation
 Bioscience Biotechnology and Biochemistry, Vol.74(4) : 788-792, 2010 
Journal Title
 Bioscience Biotechnology and Biochemistry 
ISSN
 0916-8451 
Issue Date
2010
Abstract
To evaluate the anticarcinogenic activity of methanol extract of Pleurospermum kamtschaticum (PKE), we assessed its apoptosis-inducing capability in HT-29 colon carcinoma cells. PKE treatment for 2 h reduced cell viability in a dose-related manner, and induced apoptotic morphological changes. Flow cytometric analysis indicated that PKE treatment at 0.05 mg/ml induced early apoptosis in 66.2% of HT-29 cells. Additionally, Bcl-2 expression was substantially reduced in PKE-treated HT-29 cells, increasing the Bax/Bcl-2 ratio. The protein levels of procaspase-9 and procaspase-3 were decreased markedly, reflecting caspase-9 and caspase-3 activation, and resulting PARP cleavage was noted in the PKE-treated HT-29 cells. Furthermore, we detected increased NAG-1 expression in the PKE-treated HT-29 cells. In an in vivo study, intraperitoneal PKE administration suppressed the formation of tumor nodules in the lungs of mice. These results indicate that PKE can serve as a beneficial supplement in the treatment and the prevention of colon cancer.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101140
DOI
10.1271/bbb.90826
Appears in Collections:
1. Journal Papers (연구논문) > 2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실)
1. Journal Papers (연구논문) > 5. Research Institutes (연구소) > Oral Science Research Center (구강과학연구소)
Yonsei Authors
김진은(Kim, Jin Eun) ; 박광균(Park, Kwang Kyun) ; 이창기(Lee, Chang Ki) ; 정원윤(Chung, Won Yoon)
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