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Pleurospermum kamtschaticum extract induces apoptosis via mitochondrial pathway and NAG-1 expression in colon cancer cells.

Authors
 Jin-Eun Kim  ;  Won-Yoon Chung  ;  Kyung-Soo Chun  ;  Chang Ki Lee  ;  Hee-Juhn Park  ;  Won-Bae Kim 
Citation
 BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Vol.74(4) : 788-792, 2010 
Journal Title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN
 0916-8451 
Issue Date
2010
MeSH
Apoptosis/drug effects* ; Caspase 3/metabolism ; Caspase 9/metabolism ; Cell Survival/drug effects ; Cells/metabolism ; Cells/pathology ; Colonic Neoplasms/metabolism* ; Colonic Neoplasms/pathology ; Flow Cytometry ; HT29 Cells ; Humans ; Mitochondria/metabolism* ; Mitochondria/pathology ; Poly(ADP-ribose) Polymerases/metabolism ; bcl-2-Associated X Protein/metabolism
Keywords
Pleurospermum kamtschaticum ; apoptosis ; Bax/Bcl-2 ; caspases ; NAG-1
Abstract
To evaluate the anticarcinogenic activity of methanol extract of Pleurospermum kamtschaticum (PKE), we assessed its apoptosis-inducing capability in HT-29 colon carcinoma cells. PKE treatment for 2 h reduced cell viability in a dose-related manner, and induced apoptotic morphological changes. Flow cytometric analysis indicated that PKE treatment at 0.05 mg/ml induced early apoptosis in 66.2% of HT-29 cells. Additionally, Bcl-2 expression was substantially reduced in PKE-treated HT-29 cells, increasing the Bax/Bcl-2 ratio. The protein levels of procaspase-9 and procaspase-3 were decreased markedly, reflecting caspase-9 and caspase-3 activation, and resulting PARP cleavage was noted in the PKE-treated HT-29 cells. Furthermore, we detected increased NAG-1 expression in the PKE-treated HT-29 cells. In an in vivo study, intraperitoneal PKE administration suppressed the formation of tumor nodules in the lungs of mice. These results indicate that PKE can serve as a beneficial supplement in the treatment and the prevention of colon cancer.
Files in This Item:
T201001621.pdf Download
DOI
10.1271/bbb.90826
Appears in Collections:
2. College of Dentistry (치과대학) > Research Institute (부설연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Jin Eun(김진은)
Park, Kwang Kyun(박광균)
Lee, Chang Ki(이창기)
Chung, Won Yoon(정원윤) ORCID logo https://orcid.org/0000-0001-8428-9005
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101140
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