Cited 10 times in
Pleurospermum kamtschaticum extract induces apoptosis via mitochondrial pathway and NAG-1 expression in colon cancer cells.
DC Field | Value | Language |
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dc.contributor.author | 김진은 | - |
dc.contributor.author | 박광균 | - |
dc.contributor.author | 이창기 | - |
dc.contributor.author | 정원윤 | - |
dc.date.accessioned | 2015-04-23T16:44:33Z | - |
dc.date.available | 2015-04-23T16:44:33Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0916-8451 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/101140 | - |
dc.description.abstract | To evaluate the anticarcinogenic activity of methanol extract of Pleurospermum kamtschaticum (PKE), we assessed its apoptosis-inducing capability in HT-29 colon carcinoma cells. PKE treatment for 2 h reduced cell viability in a dose-related manner, and induced apoptotic morphological changes. Flow cytometric analysis indicated that PKE treatment at 0.05 mg/ml induced early apoptosis in 66.2% of HT-29 cells. Additionally, Bcl-2 expression was substantially reduced in PKE-treated HT-29 cells, increasing the Bax/Bcl-2 ratio. The protein levels of procaspase-9 and procaspase-3 were decreased markedly, reflecting caspase-9 and caspase-3 activation, and resulting PARP cleavage was noted in the PKE-treated HT-29 cells. Furthermore, we detected increased NAG-1 expression in the PKE-treated HT-29 cells. In an in vivo study, intraperitoneal PKE administration suppressed the formation of tumor nodules in the lungs of mice. These results indicate that PKE can serve as a beneficial supplement in the treatment and the prevention of colon cancer. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 788~792 | - |
dc.relation.isPartOf | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Apoptosis/drug effects* | - |
dc.subject.MESH | Caspase 3/metabolism | - |
dc.subject.MESH | Caspase 9/metabolism | - |
dc.subject.MESH | Cell Survival/drug effects | - |
dc.subject.MESH | Cells/metabolism | - |
dc.subject.MESH | Cells/pathology | - |
dc.subject.MESH | Colonic Neoplasms/metabolism* | - |
dc.subject.MESH | Colonic Neoplasms/pathology | - |
dc.subject.MESH | Flow Cytometry | - |
dc.subject.MESH | HT29 Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mitochondria/metabolism* | - |
dc.subject.MESH | Mitochondria/pathology | - |
dc.subject.MESH | Poly(ADP-ribose) Polymerases/metabolism | - |
dc.subject.MESH | bcl-2-Associated X Protein/metabolism | - |
dc.title | Pleurospermum kamtschaticum extract induces apoptosis via mitochondrial pathway and NAG-1 expression in colon cancer cells. | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | Jin-Eun Kim | - |
dc.contributor.googleauthor | Won-Yoon Chung | - |
dc.contributor.googleauthor | Kyung-Soo Chun | - |
dc.contributor.googleauthor | Chang Ki Lee | - |
dc.contributor.googleauthor | Hee-Juhn Park | - |
dc.contributor.googleauthor | Won-Bae Kim | - |
dc.identifier.doi | 10.1271/bbb.90826 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01028 | - |
dc.contributor.localId | A01429 | - |
dc.contributor.localId | A03242 | - |
dc.contributor.localId | A03676 | - |
dc.relation.journalcode | J00328 | - |
dc.identifier.eissn | 1347-6947 | - |
dc.identifier.pmid | 20378978 | - |
dc.subject.keyword | Pleurospermum kamtschaticum | - |
dc.subject.keyword | apoptosis | - |
dc.subject.keyword | Bax/Bcl-2 | - |
dc.subject.keyword | caspases | - |
dc.subject.keyword | NAG-1 | - |
dc.contributor.alternativeName | Kim, Jin Eun | - |
dc.contributor.alternativeName | Park, Kwang Kyun | - |
dc.contributor.alternativeName | Lee, Chang Ki | - |
dc.contributor.alternativeName | Chung, Won Yoon | - |
dc.contributor.affiliatedAuthor | Kim, Jin Eun | - |
dc.contributor.affiliatedAuthor | Park, Kwang Kyun | - |
dc.contributor.affiliatedAuthor | Lee, Chang Ki | - |
dc.contributor.affiliatedAuthor | Chung, Won Yoon | - |
dc.citation.volume | 74 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 788 | - |
dc.citation.endPage | 792 | - |
dc.identifier.bibliographicCitation | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Vol.74(4) : 788-792, 2010 | - |
dc.identifier.rimsid | 52092 | - |
dc.type.rims | ART | - |
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