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Pleurospermum kamtschaticum extract induces apoptosis via mitochondrial pathway and NAG-1 expression in colon cancer cells.

DC FieldValueLanguage
dc.contributor.author김진은-
dc.contributor.author박광균-
dc.contributor.author이창기-
dc.contributor.author정원윤-
dc.date.accessioned2015-04-23T16:44:33Z-
dc.date.available2015-04-23T16:44:33Z-
dc.date.issued2010-
dc.identifier.issn0916-8451-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/101140-
dc.description.abstractTo evaluate the anticarcinogenic activity of methanol extract of Pleurospermum kamtschaticum (PKE), we assessed its apoptosis-inducing capability in HT-29 colon carcinoma cells. PKE treatment for 2 h reduced cell viability in a dose-related manner, and induced apoptotic morphological changes. Flow cytometric analysis indicated that PKE treatment at 0.05 mg/ml induced early apoptosis in 66.2% of HT-29 cells. Additionally, Bcl-2 expression was substantially reduced in PKE-treated HT-29 cells, increasing the Bax/Bcl-2 ratio. The protein levels of procaspase-9 and procaspase-3 were decreased markedly, reflecting caspase-9 and caspase-3 activation, and resulting PARP cleavage was noted in the PKE-treated HT-29 cells. Furthermore, we detected increased NAG-1 expression in the PKE-treated HT-29 cells. In an in vivo study, intraperitoneal PKE administration suppressed the formation of tumor nodules in the lungs of mice. These results indicate that PKE can serve as a beneficial supplement in the treatment and the prevention of colon cancer.-
dc.description.statementOfResponsibilityopen-
dc.format.extent788~792-
dc.relation.isPartOfBIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHApoptosis/drug effects*-
dc.subject.MESHCaspase 3/metabolism-
dc.subject.MESHCaspase 9/metabolism-
dc.subject.MESHCell Survival/drug effects-
dc.subject.MESHCells/metabolism-
dc.subject.MESHCells/pathology-
dc.subject.MESHColonic Neoplasms/metabolism*-
dc.subject.MESHColonic Neoplasms/pathology-
dc.subject.MESHFlow Cytometry-
dc.subject.MESHHT29 Cells-
dc.subject.MESHHumans-
dc.subject.MESHMitochondria/metabolism*-
dc.subject.MESHMitochondria/pathology-
dc.subject.MESHPoly(ADP-ribose) Polymerases/metabolism-
dc.subject.MESHbcl-2-Associated X Protein/metabolism-
dc.titlePleurospermum kamtschaticum extract induces apoptosis via mitochondrial pathway and NAG-1 expression in colon cancer cells.-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Oral Biology (구강생물학)-
dc.contributor.googleauthorJin-Eun Kim-
dc.contributor.googleauthorWon-Yoon Chung-
dc.contributor.googleauthorKyung-Soo Chun-
dc.contributor.googleauthorChang Ki Lee-
dc.contributor.googleauthorHee-Juhn Park-
dc.contributor.googleauthorWon-Bae Kim-
dc.identifier.doi10.1271/bbb.90826-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01028-
dc.contributor.localIdA01429-
dc.contributor.localIdA03242-
dc.contributor.localIdA03676-
dc.relation.journalcodeJ00328-
dc.identifier.eissn1347-6947-
dc.identifier.pmid20378978-
dc.subject.keywordPleurospermum kamtschaticum-
dc.subject.keywordapoptosis-
dc.subject.keywordBax/Bcl-2-
dc.subject.keywordcaspases-
dc.subject.keywordNAG-1-
dc.contributor.alternativeNameKim, Jin Eun-
dc.contributor.alternativeNamePark, Kwang Kyun-
dc.contributor.alternativeNameLee, Chang Ki-
dc.contributor.alternativeNameChung, Won Yoon-
dc.contributor.affiliatedAuthorKim, Jin Eun-
dc.contributor.affiliatedAuthorPark, Kwang Kyun-
dc.contributor.affiliatedAuthorLee, Chang Ki-
dc.contributor.affiliatedAuthorChung, Won Yoon-
dc.citation.volume74-
dc.citation.number4-
dc.citation.startPage788-
dc.citation.endPage792-
dc.identifier.bibliographicCitationBIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, Vol.74(4) : 788-792, 2010-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Oral Science Research Center (구강과학연구소) > 1. Journal Papers

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