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Doxorubicin-loaded QuadraSphere microspheres: plasma pharmacokinetics and intratumoral drug concentration in an animal model of liver cancer

Authors
 Kwang-Hun Lee  ;  Eleni A. Liapi  ;  Curt Cornell  ;  Philippe Reb  ;  Manon Buijs  ;  Josephina A. Vossen  ;  Veronica Prieto Ventura  ;  Jean-Francois H. Geschwind 
Citation
 CARDIOVASCULAR AND INTERVENTIONAL RADIOLOGY, Vol.33(3) : 576-582, 2010 
Journal Title
 CARDIOVASCULAR AND INTERVENTIONAL RADIOLOGY 
ISSN
 0174-1551 
Issue Date
2010
MeSH
Acrylic Resins ; Angiography, Digital Subtraction ; Animals ; Chemoembolization, Therapeutic/instrumentation* ; Contrast Media ; Disease Models, Animal ; Doxorubicin/administration & dosage* ; Doxorubicin/pharmacokinetics* ; Hepatic Artery ; Liver Neoplasms/drug therapy* ; Liver Neoplasms/pathology ; Male ; Microspheres* ; Polymers ; Polyvinyls ; Rabbits
Keywords
Drug-eluting microspheres ; VX-2 tumor ; Liver tumor ; Hepatic artery chemoembolization ; Superabsorbent microspheres
Abstract
The purpose of this study was to evaluate, in vitro and in vivo, doxorubicin-loaded poly (vinyl alcohol-sodium acrylate) copolymer microspheres [QuadraSphere microspheres (QSMs)] for transcatheter arterial delivery in an animal model of liver cancer. Doxorubicin loading efficiency and release profile were first tested in vitro. In vivo, 15 rabbits, implanted with a Vx-2 tumor in the liver, were divided into three groups of five rabbits each, based on the time of euthanasia. Twenty-five milligrams of QSMs was diluted in 10 ml of a 10 mg/ml doxorubicin solution and 10 ml of nonionic contrast medium for a total volume of 20 ml. One milliliter of a drug-loaded QSM solution containing 5 mg of doxorubicin was injected into the tumor feeding artery. Plasma doxorubicin and doxorubicinol concentrations, and intratumoral and peritumoral doxorubicin tissue concentrations, were measured. Tumor specimens were pathologically evaluated to record tumor necrosis. As a control, one animal was blandly embolized with plain QSMs in each group. In vitro testing of QSM doxorubicin loadability and release over time showed 82-94% doxorubicin loadability within 2 h and 6% release within the first 6 h after loading, followed by a slow release pattern. In vivo, the doxorubicin plasma concentration declined at 40 min. The peak doxorubicin intratumoral concentration was observed at 3 days and remained detectable till the study's end point (7 days). Mean percentage tumor cell death in the doxorubicin QSM group was 90% at 7 days and 60% in the bland QSM embolization group. In conclusion, QSMs can be efficiently loaded with doxorubicin. Initial experiments with doxorubicin-loaded QSMs show a safe pharmacokinetic profile and effective tumor killing in an animal model of liver cancer.
Files in This Item:
T201001616.pdf Download
DOI
10.1007/s00270-010-9794-1
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Lee, Kwang Hun(이광훈)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101132
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