Effect of Helicobacter pylori infection on the sonic hedgehog signaling pathway in gastric cancer cells

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Authors: Jie-Hyun Kim ; Yeun Jung Choi ; Sang Hun Lee ; Hyun Soo Shin ; In Ohk Lee ; Yu Jin Kim ; Hyunki Kim ; Woo Ick Yang ; Hoguen Kim ; Yong Chan Lee

MeSH: Cell Line, Tumor ; Hedgehog Proteins/genetics ; Hedgehog Proteins/metabolism* ; Helicobacter Infections/metabolism* ; Helicobacter Infections/microbiology ; Helicobacter Infections/pathology ; Helicobacter pylori/pathogenicity* ; Humans ; Immunoblotting ; Patched Receptors ; Patched-1 Receptor ; RNA, Messenger/genetics ; Receptors, Cell Surface/genetics ; Receptors, Cell Surface/metabolism* ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction ; Stomach Neoplasms/metabolism* ; Stomach Neoplasms/microbiology ; Stomach Neoplasms/pathology ; Transcription Factors/genetics ; Transcription Factors/metabolism* ; Zinc Finger Protein GLI1

Keywords: gastric cancer ; sonic hedgehog ; Helicobacter pylori ; carcinogenesis

Abstract: This study aimed to investigate the effect of Helicobacter pylori (H. pylori) infection on the sonic Hedgehog (Shh) signaling in gastric cancer. Shh, Patched (Ptch), and transcription factor Gli1 were overexpressed in H. pylori-infected gastric cancer cells. The oncoprotein, CagA positive H. pylori resulted in significantly higher Shh expression. Pretreatment with MG-132 or PDTC significantly lowered Shh expression. Significant overexpression of Shh and Gli1 were noted in H. pylori-infected compared to non-infected gastric cancer tissues. Conclusively, H. pylori activated the Shh signaling pathway in CagA-dependent manner partly through the NF-kappaB pathway in gastric cancer cells.

Appears in Collections:: 1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

Yonsei Authors: Kim, Yoo Jin(김유진)
Kim, Jie-Hyun(김지현) https://orcid.org/0000-0002-9198-3326
Kim, Hyunki(김현기) https://orcid.org/0000-0003-2292-5584
Shin, Hyun Soo(신현수)
Yang, Woo Ick(양우익) https://orcid.org/0000-0002-6084-5019
Lee, Yong Chan(이용찬) https://orcid.org/0000-0001-8800-6906
Choi, Youn Jeong(최연정)

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