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Helicobacter pylori in a Korean isolate expressed proteins differentially in human gastric epithelial cells

Authors
 Sung Hee Lee  ;  Min-Soo Lee  ;  Ji Hyun Lee  ;  So Won Kim  ;  Rhee-Hun Kang  ;  Myoung-Jin Choi  ;  Sang Jin Park  ;  Se Joo Kim  ;  Jae Myun Lee  ;  Susan P.C. Cole  ;  Min Goo Lee 
Citation
 DIGESTIVE DISEASES AND SCIENCES, Vol.55(6) : 1550-1564, 2010 
Journal Title
 DIGESTIVE DISEASES AND SCIENCES 
ISSN
 0163-2116 
Issue Date
2010
MeSH
Adenocarcinoma/microbiology ; Adenocarcinoma/pathology ; Bacterial Proteins/metabolism* ; Cell Line, Tumor ; Electrophoresis, Gel, Two-Dimensional ; Epithelial Cells/microbiology* ; Epithelial Cells/pathology ; Gastric Mucosa/microbiology* ; Gastric Mucosa/pathology ; Helicobacter Infections/epidemiology ; Helicobacter Infections/microbiology* ; Helicobacter pylori/isolation & purification ; Helicobacter pylori/metabolism ; Helicobacter pylori/pathogenicity* ; Humans ; Korea ; Proteomics/methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Stomach Neoplasms/microbiology ; Stomach Neoplasms/pathology
Keywords
Gastric epithelial cells ; Gastroduodenal disease ; Helicobacter pylori ; Proteomic analysis
Abstract
PURPOSE: The proteins expressed in gastric epithelial cells infected with Helicobacter pylori (H. pylori) may determine the clinical outcome such as chronic gastritis, peptic ulcer, and gastric carcinoma. The present study aims to determine the differentially expressed proteins in human gastric epithelial AGS cells that were infected with H. pylori in a Korean isolate, a cagA+, vacA s1b m2 iceA1 H. pylori by proteomic analysis. The differentially expressed proteins, whose expression levels were more or less than twofold in H. pylori-infected cells, were analyzed. RESULTS: Ten proteins (chromatin assembly factor-1, proliferating cell nuclear antigen, 14-3-3 protein tau, eukaryotic translation initiation factor 6, heat-shock protein 90beta, dimethylarginine dimethylaminohydrolase-1, L-lactate dehydrogenase B chain, prohibitin, triosephosphate isomerase, protein disulfide isomerase) were up-regulated while eight proteins (heat-shock gp96 precursor, nucleophosmin, ornithine aminotransferase, Ku70, L-arginine-glycine amidinotransferase, Smad anchor for receptor activation, ADP-ribosylation factor, WD repeat-containing protein isoform 1) were down-regulated by H. pylori infection in AGS cells. These proteins are related to cell proliferation, cell adhesion, carcinogenesis, cell-defense mechanisms against oxidative stress, membrane trafficking, and energy metabolism. CONCLUSIONS: Oxidative stress, cell proliferation, cell adhesion, and membrane trafficking may be involved in the pathogenesis of gastric diseases including cancer associated with H. pylori in a Korean isolate.
Full Text
http://link.springer.com/article/10.1007%2Fs10620-009-0908-z
DOI
10.1007/s10620-009-0908-z
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Hwan(김경환)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101082
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