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Chromatin organization and transcriptional activation of Hox genes

Authors
 Ji-Yeon Lee  ;  Hyehyun Min  ;  Xinnan Wang  ;  Abdul Aziz Khan  ;  Myoung Hee Kim 
Citation
 Anatomy and Cell Biology, Vol.43(1) : 78-85, 2010 
Journal Title
Anatomy and Cell Biology
ISSN
 2093-3665 
Issue Date
2010
Keywords
Hox genes ; chromosomal interaction ; chromosome conformation capture ; gene expression ; spatial organization
Abstract
Spatially and temporally programmed expression of the Hox genes along the antero-posterior (A-P) axis is essential for correct pattern formation during embryonic development. An accumulating body of evidence indicates the pivotal role of spatial chromatin organization for the coordination of gene regulation. Recently, chromosome conformation capture (3C) technique has been developed and opened a new way to study chromosomal interactions in the nucleus. In this study, we describe 3C method we applied in F9 embryonic teratocarcinoma cells and demonstrate that the chromosomal interactions at Hox loci are successfully detected. Interestingly, at Hoxc loci, the abundance of intrachromosomal interactions with neighboring fragments was drastically decreased when the genes are expressed. These results indicate the possibility of the dynamic pattern of chromosomal interaction in association with the transcriptional regulation of Hox genes
Files in This Item:
T201001164.pdf Download
DOI
10.5115/acb.2010.43.1.78
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Kim, Myoung Hee(김명희) ORCID logo https://orcid.org/0000-0001-5652-1452
Min, Hye Hyun(민혜현) ORCID logo https://orcid.org/0000-0001-5450-5088
Lee, Ji Yeon(이지연) ORCID logo https://orcid.org/0000-0002-0670-3095
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/101003
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