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CD24+ cells from hierarchically organized ovarian cancer are enriched in cancer stem cells

 M-Q Gao  ;  Y-P Choi  ;  S Kang  ;  J H Youn  ;  N-H Cho 
 ONCOGENE, Vol.29(18) : 2672-2680, 2010 
Journal Title
Issue Date
AC133 Antigen ; Animals ; Antigens, CD/analysis ; CD24 Antigen/analysis* ; Female ; Glycoproteins/analysis ; Humans ; Mice ; Neoplastic Stem Cells/pathology* ; Ovarian Neoplasms/chemistry ; Ovarian Neoplasms/pathology* ; Peptides/analysis ; Proto-Oncogene Proteins c-kit/analysis
ovarian cancer ; cancer stem cell ; tumorigenicity
Cancer stem cells (CSCs) have been identified in solid tumors and cancer cell lines. In this study, we isolated a series of cancer cell clones, which were heterogeneous in growth rate, cell cycle distribution and expression profile of genes and proteins, from ovarian tumor specimens of a patient and identified a sub-population enriched for ovarian CSCs defined by CD24 phenotype. Experiments in vitro demonstrated CD24(+) sub-population possessed stem cell-like characteristics of remaining quiescence and more chemoresistant compared with CD24(-) fraction, as well as a specific capacity for self-renewal and differentiation. In addition, injection of 5 x 10(3) CD24(+) cells was able to form tumor xenografts in nude mice, whereas equal number of CD24(-) cells remained nontumorigenic. We also found that CD24(+) cells expressed higher mRNA levels of some 'stemness' genes, including Nestin, beta-catenin, Bmi-1, Oct4, Oct3/4, Notch1 and Notch4 which were involved in modulating many functions of stem cells, and lower E-cadherin mRNA level than CD24(-) cells. Altogether, these observations suggest human ovarian tumor cells are organized as a hierarchy and CD24 demarcates an ovarian cancer-initiating cell population. These findings will have important clinical applications for developing effective therapeutic strategies to treat ovarian cancer.
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1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Suki(강숙희) ORCID logo https://orcid.org/0000-0002-9957-3479
Gao, Ming Qing(고명청)
Yoon, Joo Heon(윤주헌)
Cho, Nam Hoon(조남훈) ORCID logo https://orcid.org/0000-0002-0045-6441
Choi, Yoon Pyo(최윤표)
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