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Neuroprotective effects of FK506 against excitotoxicity in organotypic hippocampal slice culture

Authors
 Kyung Hee Lee  ;  Ran Won  ;  Un Jeng Kim  ;  Ga Min Kim  ;  Myung-Ae Chung  ;  Jin-Hun Sohn  ;  Bae Hwan Lee 
Citation
 NEUROSCIENCE LETTERS, Vol.474(3) : 126-130, 2010 
Journal Title
NEUROSCIENCE LETTERS
ISSN
 0304-3940 
Issue Date
2010
MeSH
Animals ; Blotting, Western ; Cell Death/drug effects ; Excitatory Amino Acid Agonists/toxicity ; Hippocampus/drug effects ; Kainic Acid/toxicity ; Neurons/drug effects* ; Neuroprotective Agents/pharmacology* ; Organ Culture Techniques ; Rats ; Rats, Sprague-Dawley ; Tacrolimus/pharmacology*
Keywords
FK506 ; Kainic acid ; Organotypic hippocampal slice culture ; Neuroprotection
Abstract
FK506 has been originally classified as an immunosuppressant and is known to exhibit neurotrophic actions in vitro and protective effects on some neurological conditions. We investigated the neuroprotective effects of FK506 on kainic acid (KA)-induced neuronal death in organotypic hippocampal slice cultures (OHSCs). After an 18 h KA (5 microM) treatment, significantly neuronal death was detected in the CA3 region using propidium iodide staining. However, neuronal death was significantly prevented at 24 and 48 h after treatment with 0.1 microM FK506. Using cresyl violet staining, we also observed that an increased number of CA3 neurons survived in the 0.1 microM FK506 group compared to the KA only group. Based on the results of the Western blot analysis, the expressions of 5-lipoxygenase and caspase-3 were reduced 24h after 0.1 microM FK506 treatment. The levels of superoxide dismutase (SOD) and phospho-Akt expression were increased by treatment with 0.1 microM FK506. These results suggest that FK506 may have a positive role in protecting neurons against cell death in the KA injury model of OHSCs
Full Text
http://www.sciencedirect.com/science/article/pii/S030439401000282X
DOI
10.1016/j.neulet.2010.03.009
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kim, Ga Min(김가민)
Kim, Un Jeng(김은정) ORCID logo https://orcid.org/0000-0003-0968-0252
Lee, Bae Hwan(이배환) ORCID logo https://orcid.org/0000-0003-4719-9021
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100895
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