Cited 14 times in
Neuroprotective effects of FK506 against excitotoxicity in organotypic hippocampal slice culture
DC Field | Value | Language |
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dc.contributor.author | 김가민 | - |
dc.contributor.author | 김은정 | - |
dc.contributor.author | 이배환 | - |
dc.date.accessioned | 2015-04-23T16:36:35Z | - |
dc.date.available | 2015-04-23T16:36:35Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0304-3940 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/100895 | - |
dc.description.abstract | FK506 has been originally classified as an immunosuppressant and is known to exhibit neurotrophic actions in vitro and protective effects on some neurological conditions. We investigated the neuroprotective effects of FK506 on kainic acid (KA)-induced neuronal death in organotypic hippocampal slice cultures (OHSCs). After an 18 h KA (5 microM) treatment, significantly neuronal death was detected in the CA3 region using propidium iodide staining. However, neuronal death was significantly prevented at 24 and 48 h after treatment with 0.1 microM FK506. Using cresyl violet staining, we also observed that an increased number of CA3 neurons survived in the 0.1 microM FK506 group compared to the KA only group. Based on the results of the Western blot analysis, the expressions of 5-lipoxygenase and caspase-3 were reduced 24h after 0.1 microM FK506 treatment. The levels of superoxide dismutase (SOD) and phospho-Akt expression were increased by treatment with 0.1 microM FK506. These results suggest that FK506 may have a positive role in protecting neurons against cell death in the KA injury model of OHSCs | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | NEUROSCIENCE LETTERS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Cell Death/drug effects | - |
dc.subject.MESH | Excitatory Amino Acid Agonists/toxicity | - |
dc.subject.MESH | Hippocampus/drug effects | - |
dc.subject.MESH | Kainic Acid/toxicity | - |
dc.subject.MESH | Neurons/drug effects* | - |
dc.subject.MESH | Neuroprotective Agents/pharmacology* | - |
dc.subject.MESH | Organ Culture Techniques | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Tacrolimus/pharmacology* | - |
dc.title | Neuroprotective effects of FK506 against excitotoxicity in organotypic hippocampal slice culture | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Medical Research Center (임상의학연구센터) | - |
dc.contributor.googleauthor | Kyung Hee Lee | - |
dc.contributor.googleauthor | Ran Won | - |
dc.contributor.googleauthor | Un Jeng Kim | - |
dc.contributor.googleauthor | Ga Min Kim | - |
dc.contributor.googleauthor | Myung-Ae Chung | - |
dc.contributor.googleauthor | Jin-Hun Sohn | - |
dc.contributor.googleauthor | Bae Hwan Lee | - |
dc.identifier.doi | 10.1016/j.neulet.2010.03.009 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02662 | - |
dc.contributor.localId | A00285 | - |
dc.contributor.localId | A00817 | - |
dc.contributor.localId | A02791 | - |
dc.relation.journalcode | J02364 | - |
dc.identifier.eissn | 1872-7972 | - |
dc.identifier.pmid | 20226231 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S030439401000282X | - |
dc.subject.keyword | FK506 | - |
dc.subject.keyword | Kainic acid | - |
dc.subject.keyword | Organotypic hippocampal slice culture | - |
dc.subject.keyword | Neuroprotection | - |
dc.contributor.alternativeName | Kim, Ga Min | - |
dc.contributor.alternativeName | Kim, Un Jeng | - |
dc.contributor.alternativeName | Lee, Bae Hwan | - |
dc.contributor.affiliatedAuthor | Kim, Ga Min | - |
dc.contributor.affiliatedAuthor | Kim, Un Jeng | - |
dc.contributor.affiliatedAuthor | Lee, Bae Hwan | - |
dc.citation.volume | 474 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 126 | - |
dc.citation.endPage | 130 | - |
dc.identifier.bibliographicCitation | NEUROSCIENCE LETTERS, Vol.474(3) : 126-130, 2010 | - |
dc.identifier.rimsid | 55628 | - |
dc.type.rims | ART | - |
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