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Neuroprotective effects of FK506 against excitotoxicity in organotypic hippocampal slice culture

DC Field Value Language
dc.contributor.author김가민-
dc.contributor.author김은정-
dc.contributor.author이경희-
dc.contributor.author이배환-
dc.date.accessioned2015-04-23T16:36:35Z-
dc.date.available2015-04-23T16:36:35Z-
dc.date.issued2010-
dc.identifier.issn0304-3940-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/100895-
dc.description.abstractFK506 has been originally classified as an immunosuppressant and is known to exhibit neurotrophic actions in vitro and protective effects on some neurological conditions. We investigated the neuroprotective effects of FK506 on kainic acid (KA)-induced neuronal death in organotypic hippocampal slice cultures (OHSCs). After an 18 h KA (5 microM) treatment, significantly neuronal death was detected in the CA3 region using propidium iodide staining. However, neuronal death was significantly prevented at 24 and 48 h after treatment with 0.1 microM FK506. Using cresyl violet staining, we also observed that an increased number of CA3 neurons survived in the 0.1 microM FK506 group compared to the KA only group. Based on the results of the Western blot analysis, the expressions of 5-lipoxygenase and caspase-3 were reduced 24h after 0.1 microM FK506 treatment. The levels of superoxide dismutase (SOD) and phospho-Akt expression were increased by treatment with 0.1 microM FK506. These results suggest that FK506 may have a positive role in protecting neurons against cell death in the KA injury model of OHSCs-
dc.description.statementOfResponsibilityopen-
dc.format.extent126~130-
dc.relation.isPartOfNEUROSCIENCE LETTERS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCell Death/drug effects-
dc.subject.MESHExcitatory Amino Acid Agonists/toxicity-
dc.subject.MESHHippocampus/drug effects-
dc.subject.MESHKainic Acid/toxicity-
dc.subject.MESHNeurons/drug effects*-
dc.subject.MESHNeuroprotective Agents/pharmacology*-
dc.subject.MESHOrgan Culture Techniques-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHTacrolimus/pharmacology*-
dc.titleNeuroprotective effects of FK506 against excitotoxicity in organotypic hippocampal slice culture-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentMedical Research Center (임상의학연구센터)-
dc.contributor.googleauthorKyung Hee Lee-
dc.contributor.googleauthorRan Won-
dc.contributor.googleauthorUn Jeng Kim-
dc.contributor.googleauthorGa Min Kim-
dc.contributor.googleauthorMyung-Ae Chung-
dc.contributor.googleauthorJin-Hun Sohn-
dc.contributor.googleauthorBae Hwan Lee-
dc.identifier.doi10.1016/j.neulet.2010.03.009-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02662-
dc.contributor.localIdA00285-
dc.contributor.localIdA00817-
dc.contributor.localIdA02791-
dc.relation.journalcodeJ02364-
dc.identifier.eissn1872-7972-
dc.identifier.pmid20226231-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S030439401000282X-
dc.subject.keywordFK506-
dc.subject.keywordKainic acid-
dc.subject.keywordOrganotypic hippocampal slice culture-
dc.subject.keywordNeuroprotection-
dc.contributor.alternativeNameKim, Ga Min-
dc.contributor.alternativeNameKim, Un Jeng-
dc.contributor.alternativeNameLee, Kyung Hee-
dc.contributor.alternativeNameLee, Bae Hwan-
dc.contributor.affiliatedAuthorLee, Kyung Hee-
dc.contributor.affiliatedAuthorKim, Ga Min-
dc.contributor.affiliatedAuthorKim, Un Jeng-
dc.contributor.affiliatedAuthorLee, Bae Hwan-
dc.citation.volume474-
dc.citation.number3-
dc.citation.startPage126-
dc.citation.endPage130-
dc.identifier.bibliographicCitationNEUROSCIENCE LETTERS, Vol.474(3) : 126-130, 2010-
dc.identifier.rimsid55628-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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