1 286

Cited 19 times in

House dust mite extract activates apical Cl(-) channels through protease-activated receptor 2 in human airway epithelia

DC FieldValueLanguage
dc.contributor.author최재영-
dc.contributor.author홍정희-
dc.contributor.author김창훈-
dc.contributor.author신동민-
dc.contributor.author윤주헌-
dc.contributor.author이현재-
dc.contributor.author지헌영-
dc.date.accessioned2015-04-23T16:35:10Z-
dc.date.available2015-04-23T16:35:10Z-
dc.date.issued2010-
dc.identifier.issn0730-2312-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/100852-
dc.description.abstractAdequate fluid secretion from airway mucosa is essential for maintaining mucociliary clearance, and fluid hypersecretion is a prominent feature of inflammatory airway diseases such as allergic rhinitis. House dust mite extract (HDM) has been reported to activate protease-activated receptors (PARs), which play various roles in airway epithelia. However, the role of HDM in regulating ion transporters and fluid secretion has not been investigated. We examined the effect of HDM on ion transport in human primary nasal epithelial cells. The Ca(2+)-sensitive dye Fura2-AM was used to determine intracellular Ca(2+) concentration ([Ca(2+)](i)) by means of spectrofluorometry in human normal nasal epithelial cells (NHNE). Short-circuit current (Isc) was measured using Ussing chambers. Fluid secretion from porcine airway mucosa was observed by optical measurement. HDM extract (10 microg/Ml) effectively cleaved the PAR-2 peptide and induced an increase of [Ca(2+)](i) that was abolished by desensitization with trypsin, but not with thrombin. Apical application of HDM-induced Isc sensitive to both a cystic fibrosis transmembrane conductance regulator (CFTR) inhibitor and a Ca(2+)-activated Cl(-) channel (CaCC) inhibitor. HDM extract also stimulated fluid secretion from porcine airway mucosa. HDM extract activated PAR-2 and apical Cl(-) secretion via CaCC and CFTR, and HDM-induced fluid secretion in porcine airway mucosa. Our results suggest a role for PAR-2 in mucociliary clearance and fluid hypersecretion of airway mucosa in response to air-borne allergens such as HDM.-
dc.description.statementOfResponsibilityopen-
dc.format.extent1254~1263-
dc.relation.isPartOfJOURNAL OF CELLULAR BIOCHEMISTRY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAllergens/pharmacology*-
dc.subject.MESHAnimals-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCalcium/metabolism-
dc.subject.MESHCell Line-
dc.subject.MESHCells, Cultured-
dc.subject.MESHChloride Channels/drug effects*-
dc.subject.MESHEpithelial Cells/cytology-
dc.subject.MESHEpithelial Cells/drug effects*-
dc.subject.MESHEpithelial Cells/metabolism*-
dc.subject.MESHHumans-
dc.subject.MESHPatch-Clamp Techniques-
dc.subject.MESHPyroglyphidae/chemistry*-
dc.subject.MESHReceptor, PAR-2/metabolism*-
dc.subject.MESHRespiratory Mucosa/cytology*-
dc.subject.MESHReverse Transcriptase Polymerase Chain Reaction-
dc.subject.MESHRhinitis/metabolism-
dc.subject.MESHRhinitis, Allergic, Seasonal/metabolism-
dc.titleHouse dust mite extract activates apical Cl(-) channels through protease-activated receptor 2 in human airway epithelia-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentYonsei Biomedical Research Center (연세의생명연구원)-
dc.contributor.googleauthorHyung-Ju Cho-
dc.contributor.googleauthorJae Young Choi-
dc.contributor.googleauthorYu-Mi Yang-
dc.contributor.googleauthorJeong Hee Hong-
dc.contributor.googleauthorChang-Hoon Kim-
dc.contributor.googleauthorHeon Young Gee-
dc.contributor.googleauthorHyun Jae Lee-
dc.contributor.googleauthorDong Min Shin-
dc.contributor.googleauthorJoo-Heon Yoon-
dc.identifier.doi10.1002/jcb.22511-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA04434-
dc.contributor.localIdA02091-
dc.contributor.localIdA02604-
dc.contributor.localIdA03293-
dc.contributor.localIdA03971-
dc.contributor.localIdA04173-
dc.contributor.localIdA01050-
dc.relation.journalcodeJ01303-
dc.identifier.eissn1097-4644-
dc.identifier.pmid20186875-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1002/jcb.22511/abstract-
dc.subject.keywordHOUSE DUST MITES-
dc.subject.keywordSECRETION-
dc.subject.keywordION CHANNEL-
dc.subject.keywordPROTEASE-ACTIVATED RECEPTORS-
dc.subject.keywordNASAL EPIT HELIUM-
dc.contributor.alternativeNameChoi, Jae Young-
dc.contributor.alternativeNameHong, Jeong Hee-
dc.contributor.alternativeNameKim, Chang Hoon-
dc.contributor.alternativeNameShin, Dong Min-
dc.contributor.alternativeNameYoon, Joo Heon-
dc.contributor.alternativeNameLee, Hyun Jae-
dc.contributor.alternativeNameGee, Heon Yung-
dc.contributor.affiliatedAuthorHong, Jeong Hee-
dc.contributor.affiliatedAuthorShin, Dong Min-
dc.contributor.affiliatedAuthorYoon, Joo Heon-
dc.contributor.affiliatedAuthorLee, Hyun Jae-
dc.contributor.affiliatedAuthorGee, Heon Yung-
dc.contributor.affiliatedAuthorChoi, Jae Young-
dc.contributor.affiliatedAuthorKim, Chang Hoon-
dc.citation.volume109-
dc.citation.number6-
dc.citation.startPage1254-
dc.citation.endPage1263-
dc.identifier.bibliographicCitationJOURNAL OF CELLULAR BIOCHEMISTRY, Vol.109(6) : 1254-1263, 2010-
Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.