Cited 27 times in
House dust mite extract activates apical Cl(-) channels through protease-activated receptor 2 in human airway epithelia
DC Field | Value | Language |
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dc.contributor.author | 최재영 | - |
dc.contributor.author | 홍정희 | - |
dc.contributor.author | 김창훈 | - |
dc.contributor.author | 신동민 | - |
dc.contributor.author | 윤주헌 | - |
dc.contributor.author | 이현재 | - |
dc.contributor.author | 지헌영 | - |
dc.date.accessioned | 2015-04-23T16:35:10Z | - |
dc.date.available | 2015-04-23T16:35:10Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0730-2312 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/100852 | - |
dc.description.abstract | Adequate fluid secretion from airway mucosa is essential for maintaining mucociliary clearance, and fluid hypersecretion is a prominent feature of inflammatory airway diseases such as allergic rhinitis. House dust mite extract (HDM) has been reported to activate protease-activated receptors (PARs), which play various roles in airway epithelia. However, the role of HDM in regulating ion transporters and fluid secretion has not been investigated. We examined the effect of HDM on ion transport in human primary nasal epithelial cells. The Ca(2+)-sensitive dye Fura2-AM was used to determine intracellular Ca(2+) concentration ([Ca(2+)](i)) by means of spectrofluorometry in human normal nasal epithelial cells (NHNE). Short-circuit current (Isc) was measured using Ussing chambers. Fluid secretion from porcine airway mucosa was observed by optical measurement. HDM extract (10 microg/Ml) effectively cleaved the PAR-2 peptide and induced an increase of [Ca(2+)](i) that was abolished by desensitization with trypsin, but not with thrombin. Apical application of HDM-induced Isc sensitive to both a cystic fibrosis transmembrane conductance regulator (CFTR) inhibitor and a Ca(2+)-activated Cl(-) channel (CaCC) inhibitor. HDM extract also stimulated fluid secretion from porcine airway mucosa. HDM extract activated PAR-2 and apical Cl(-) secretion via CaCC and CFTR, and HDM-induced fluid secretion in porcine airway mucosa. Our results suggest a role for PAR-2 in mucociliary clearance and fluid hypersecretion of airway mucosa in response to air-borne allergens such as HDM. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1254~1263 | - |
dc.relation.isPartOf | JOURNAL OF CELLULAR BIOCHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Allergens/pharmacology* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Calcium/metabolism | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Chloride Channels/drug effects* | - |
dc.subject.MESH | Epithelial Cells/cytology | - |
dc.subject.MESH | Epithelial Cells/drug effects* | - |
dc.subject.MESH | Epithelial Cells/metabolism* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Patch-Clamp Techniques | - |
dc.subject.MESH | Pyroglyphidae/chemistry* | - |
dc.subject.MESH | Receptor, PAR-2/metabolism* | - |
dc.subject.MESH | Respiratory Mucosa/cytology* | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Rhinitis/metabolism | - |
dc.subject.MESH | Rhinitis, Allergic, Seasonal/metabolism | - |
dc.title | House dust mite extract activates apical Cl(-) channels through protease-activated receptor 2 in human airway epithelia | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.googleauthor | Hyung-Ju Cho | - |
dc.contributor.googleauthor | Jae Young Choi | - |
dc.contributor.googleauthor | Yu-Mi Yang | - |
dc.contributor.googleauthor | Jeong Hee Hong | - |
dc.contributor.googleauthor | Chang-Hoon Kim | - |
dc.contributor.googleauthor | Heon Young Gee | - |
dc.contributor.googleauthor | Hyun Jae Lee | - |
dc.contributor.googleauthor | Dong Min Shin | - |
dc.contributor.googleauthor | Joo-Heon Yoon | - |
dc.identifier.doi | 10.1002/jcb.22511 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04434 | - |
dc.contributor.localId | A02091 | - |
dc.contributor.localId | A02604 | - |
dc.contributor.localId | A03293 | - |
dc.contributor.localId | A03971 | - |
dc.contributor.localId | A04173 | - |
dc.contributor.localId | A01050 | - |
dc.relation.journalcode | J01303 | - |
dc.identifier.eissn | 1097-4644 | - |
dc.identifier.pmid | 20186875 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1002/jcb.22511/abstract | - |
dc.subject.keyword | HOUSE DUST MITES | - |
dc.subject.keyword | SECRETION | - |
dc.subject.keyword | ION CHANNEL | - |
dc.subject.keyword | PROTEASE-ACTIVATED RECEPTORS | - |
dc.subject.keyword | NASAL EPIT HELIUM | - |
dc.contributor.alternativeName | Choi, Jae Young | - |
dc.contributor.alternativeName | Hong, Jeong Hee | - |
dc.contributor.alternativeName | Kim, Chang Hoon | - |
dc.contributor.alternativeName | Shin, Dong Min | - |
dc.contributor.alternativeName | Yoon, Joo Heon | - |
dc.contributor.alternativeName | Lee, Hyun Jae | - |
dc.contributor.alternativeName | Gee, Heon Yung | - |
dc.contributor.affiliatedAuthor | Hong, Jeong Hee | - |
dc.contributor.affiliatedAuthor | Shin, Dong Min | - |
dc.contributor.affiliatedAuthor | Yoon, Joo Heon | - |
dc.contributor.affiliatedAuthor | Lee, Hyun Jae | - |
dc.contributor.affiliatedAuthor | Gee, Heon Yung | - |
dc.contributor.affiliatedAuthor | Choi, Jae Young | - |
dc.contributor.affiliatedAuthor | Kim, Chang Hoon | - |
dc.citation.volume | 109 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 1254 | - |
dc.citation.endPage | 1263 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CELLULAR BIOCHEMISTRY, Vol.109(6) : 1254-1263, 2010 | - |
dc.identifier.rimsid | 55261 | - |
dc.type.rims | ART | - |
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