1 328

Cited 10 times in

Proliferating cell nuclear antigen (Pcna) as a direct downstream target gene of Hoxc8

Authors
 Hyehyun Min  ;  Ji-Yeon Lee  ;  Jinwoong Bok  ;  Hyun Joo Chung  ;  Myoung Hee Kim 
Citation
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.392(4) : 543-547, 2010 
Journal Title
 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 
ISSN
 0006-291X 
Issue Date
2010
MeSH
Animals ; Base Sequence ; Cells, Cultured ; Chromatin Immunoprecipitation ; Embryonic Development/genetics* ; Gene Expression Regulation, Developmental* ; Homeodomain Proteins/genetics ; Homeodomain Proteins/metabolism* ; Mice ; Mice, Inbred ICR ; Molecular Sequence Data ; Proliferating Cell Nuclear Antigen/genetics* ; Transcription, Genetic
Keywords
Hoxc8 ; Proliferating cell nuclear antigen ; Embryonic development
Abstract
Hoxc8 is a member of Hox family transcription factors that play crucial roles in spatiotemporal body patterning during embryogenesis. Hox proteins contain a conserved 61 amino acid homeodomain, which is responsible for recognition and binding of the proteins onto Hox-specific DNA binding motifs and regulates expression of their target genes. Previously, using proteome analysis, we identified Proliferating cell nuclear antigen (Pcna) as one of the putative target genes of Hoxc8. Here, we asked whether Hoxc8 regulates Pcna expression by directly binding to the regulatory sequence of Pcna. In mouse embryos at embryonic day 11.5, the expression pattern of Pcna was similar to that of Hoxc8 along the anteroposterior body axis. Moreover, Pcna transcript levels as well as cell proliferation rate were increased by overexpression of Hoxc8 in C3H10T1/2 mouse embryonic fibroblast cells. Characterization of 2.3kb genomic sequence upstream of Pcna coding region revealed that the upstream sequence contains several Hox core binding sequences and one Hox-Pbx binding sequence. Direct binding of Hoxc8 proteins to the Pcna regulatory sequence was verified by chromatin immunoprecipitation assay. Taken together, our data suggest that Pcna is a direct downstream target of Hoxc8.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X10001087
DOI
10.1016/j.bbrc.2010.01.059
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
Yonsei Authors
Kim, Myoung Hee(김명희) ORCID logo https://orcid.org/0000-0001-5652-1452
Min, Hye Hyun(민혜현) ORCID logo https://orcid.org/0000-0001-5450-5088
Bok, Jin Woong(복진웅) ORCID logo https://orcid.org/0000-0003-1958-1872
Lee, Ji Yeon(이지연) ORCID logo https://orcid.org/0000-0002-0670-3095
Chung, Huyun Joo(정현주)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/100605
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links