2 241

Cited 8 times in

Ca2+ signaling induced by sphingosine 1-phosphate and lysophosphatidic acid in mouse B cells

 Joo Hyun Nam  ;  Dong Hun Shin  ;  Jung Eun Min  ;  Sang-Kyu Ye  ;  Ju-Hong Jeon  ;  Sung Joon Kim 
 MOLECULES AND CELLS, Vol.29(1) : 85-91, 2010 
Journal Title
Issue Date
Animals ; B-Lymphocytes/drug effects* ; B-Lymphocytes/immunology ; B-Lymphocytes/metabolism ; B-Lymphocytes/pathology ; Bone Marrow/pathology ; Calcium Signaling/drug effects ; Calcium Signaling/immunology ; Calcium-Transporting ATPases/genetics ; Calcium-Transporting ATPases/immunology ; Calcium-Transporting ATPases/metabolism* ; Cell Culture Techniques ; Cell Line ; Lipid Metabolism/immunology ; Lymphocyte Activation ; Lymphopoiesis ; Lysophospholipids/pharmacology* ; Mice ; Precursor Cells, B-Lymphoid/drug effects* ; Precursor Cells, B-Lymphoid/immunology ; Precursor Cells, B-Lymphoid/metabolism ; Precursor Cells, B-Lymphoid/pathology ; Sphingosine/analogs & derivatives* ; Sphingosine/pharmacology ; Spleen/pathology ; Thapsigargin/pharmacology ; Type C Phospholipases/metabolism
B cell ; Ca2+ signal ; lysophosphatidic acid ; sphingosine 1-phosphate ; store-operated Ca2+ influx
Lysophospholipids (LPLs) such as lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) are chemotactic for lymphocytes, and increases of in cytosolic [Ca(2+)] signal the regulation of lymphocyte activation and migration. Here, the authors investigated the effects of LPA and S1P on [Ca(2+)](c) in mouse B cell lines (WEHI-231 and Bal-17) and primary B cells isolated from mouse spleen and bone marrow, and focused on the modulation of store-operated Ca(2+) entry (SOCE) by LPLs. In Bal-17 (a mature B cell line) both LPA and S1P induced a transient [Ca(2+)](c) increase via a phospholipase C pathway. In addition, pretreatment with LPLs was found to augment thapsigargin-induced SOCE in Bal-17 cells. However, in WEHI-231 (an immature B cell line) LPLs had no significant effect on [Ca(2+)](c) or SOCE. Furthermore, in freshly isolated splenic B cells (SBCs) and bone marrow B cells (BMBCs), LPLs induced only a small increase in [Ca(2+)](c). Interestingly, however, pretreatment with LPLs markedly increased SOCE in primary B cells, and this augmentation was more prominent in BMBCs than SBCs. The unidirectional influx of Ca(2+) was measured using Ba(2+) as a surrogate ion. Similarly, Ba(2+) influx was also found to be markedly increased by LPLs in SBCs and BMBCs. Summarizing, LPLs were found to strongly augment SOCE-mediated Ca(2+)-signaling in mouse B cells. However, unlike the mature Bal-17 cell line, PLC-dependent Ca(2+) release was insignificant in primary B cells and inWEHI-231.
Full Text
Appears in Collections:
5. Research Institutes (연구소) > Research Center for Human Natural Defense System (생체방어연구센터) > 1. Journal Papers
Yonsei Authors
Nam, Joo Hyun(남주현)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.