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DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B elicits polyfunctional CD8+ T cell responses

 Jino Lee  ;  Jihye Kim  ;  Jeewon Lee  ;  Sung Jae Shin  ;  Eui-Cheol Shin 
 Clinical Experimental Vaccine Research, Vol.3(2) : 235-243, 2014 
Journal Title
 Clinical Experimental Vaccine Research 
Issue Date
DNA vaccines ; Polyfunctional T cells ; Resuscitation-promoting factor B ; Tuberculosis
PURPOSE: T cell-mediated immune responses, and particularly activation of polyfunctional T cells that simultaneously produce multiple cytokines, are necessary for the control of Mycobacterium tuberculosis. In the present study, we examined if DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B (RpfB) elicits polyfunctional T cell responses in mice. MATERIALS AND METHODS: C57BL/6 mice were immunized intramuscularly three times, at 3-week intervals, with RpfB-expressing plasmid DNA. For comparison, protein immunization was performed with recombinant RpfB in control mice. After immunization, RpfB-specific T cell responses were assessed by interferon-γ (IFN-γ) enzyme-linked immunosorbent spot assay and intracellular cytokine staining (ICS), and T cell polyfunctionality was assessed from the ICS data. RESULTS: RpfB DNA immunization induced not only humoral immune responses, but also CD8(+) and CD4(+) T cell responses. Immunodominant T-cell epitopes were identified within RpfB by assays with overlapping peptides. RpfB DNA immunization elicited a polyfunctional CD8(+) T cell response that was dominated by a functional phenotype of IFN-γ(+)/TNF-α(+)/IL-2(-)/CD107a(+). CONCLUSION: RpfB DNA immunization elicits polyfunctional CD8(+) T cell responses, suggesting that RpfB DNA immunization might induce protective immunity against tuberculosis.
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1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Shin, Sung Jae(신성재) ORCID logo https://orcid.org/0000-0003-0854-4582
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