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DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B elicits polyfunctional CD8+ T cell responses

DC FieldValueLanguage
dc.contributor.author신성재-
dc.date.accessioned2015-01-06T17:23:57Z-
dc.date.available2015-01-06T17:23:57Z-
dc.date.issued2014-
dc.identifier.issn2287-3651-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/99905-
dc.description.abstractPURPOSE: T cell-mediated immune responses, and particularly activation of polyfunctional T cells that simultaneously produce multiple cytokines, are necessary for the control of Mycobacterium tuberculosis. In the present study, we examined if DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B (RpfB) elicits polyfunctional T cell responses in mice. MATERIALS AND METHODS: C57BL/6 mice were immunized intramuscularly three times, at 3-week intervals, with RpfB-expressing plasmid DNA. For comparison, protein immunization was performed with recombinant RpfB in control mice. After immunization, RpfB-specific T cell responses were assessed by interferon-γ (IFN-γ) enzyme-linked immunosorbent spot assay and intracellular cytokine staining (ICS), and T cell polyfunctionality was assessed from the ICS data. RESULTS: RpfB DNA immunization induced not only humoral immune responses, but also CD8(+) and CD4(+) T cell responses. Immunodominant T-cell epitopes were identified within RpfB by assays with overlapping peptides. RpfB DNA immunization elicited a polyfunctional CD8(+) T cell response that was dominated by a functional phenotype of IFN-γ(+)/TNF-α(+)/IL-2(-)/CD107a(+). CONCLUSION: RpfB DNA immunization elicits polyfunctional CD8(+) T cell responses, suggesting that RpfB DNA immunization might induce protective immunity against tuberculosis.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.relation.isPartOfClinical Experimental Vaccine Research-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleDNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B elicits polyfunctional CD8+ T cell responses-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorJino Lee-
dc.contributor.googleauthorJihye Kim-
dc.contributor.googleauthorJeewon Lee-
dc.contributor.googleauthorSung Jae Shin-
dc.contributor.googleauthorEui-Cheol Shin-
dc.identifier.doi10.7774/cevr.2014.3.2.235-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02113-
dc.relation.journalcodeJ00573-
dc.identifier.pmid25003098-
dc.subject.keywordDNA vaccines-
dc.subject.keywordPolyfunctional T cells-
dc.subject.keywordResuscitation-promoting factor B-
dc.subject.keywordTuberculosis-
dc.contributor.alternativeNameShin, Sung Jae-
dc.contributor.affiliatedAuthorShin, Sung Jae-
dc.citation.volume3-
dc.citation.number2-
dc.citation.startPage235-
dc.citation.endPage243-
dc.identifier.bibliographicCitationClinical Experimental Vaccine Research, Vol.3(2) : 235-243, 2014-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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