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DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B elicits polyfunctional CD8+ T cell responses
DC Field | Value | Language |
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dc.contributor.author | 신성재 | - |
dc.date.accessioned | 2015-01-06T17:23:57Z | - |
dc.date.available | 2015-01-06T17:23:57Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 2287-3651 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/99905 | - |
dc.description.abstract | PURPOSE: T cell-mediated immune responses, and particularly activation of polyfunctional T cells that simultaneously produce multiple cytokines, are necessary for the control of Mycobacterium tuberculosis. In the present study, we examined if DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B (RpfB) elicits polyfunctional T cell responses in mice. MATERIALS AND METHODS: C57BL/6 mice were immunized intramuscularly three times, at 3-week intervals, with RpfB-expressing plasmid DNA. For comparison, protein immunization was performed with recombinant RpfB in control mice. After immunization, RpfB-specific T cell responses were assessed by interferon-γ (IFN-γ) enzyme-linked immunosorbent spot assay and intracellular cytokine staining (ICS), and T cell polyfunctionality was assessed from the ICS data. RESULTS: RpfB DNA immunization induced not only humoral immune responses, but also CD8(+) and CD4(+) T cell responses. Immunodominant T-cell epitopes were identified within RpfB by assays with overlapping peptides. RpfB DNA immunization elicited a polyfunctional CD8(+) T cell response that was dominated by a functional phenotype of IFN-γ(+)/TNF-α(+)/IL-2(-)/CD107a(+). CONCLUSION: RpfB DNA immunization elicits polyfunctional CD8(+) T cell responses, suggesting that RpfB DNA immunization might induce protective immunity against tuberculosis. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | Clinical Experimental Vaccine Research | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | DNA immunization of Mycobacterium tuberculosis resuscitation-promoting factor B elicits polyfunctional CD8+ T cell responses | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Jino Lee | - |
dc.contributor.googleauthor | Jihye Kim | - |
dc.contributor.googleauthor | Jeewon Lee | - |
dc.contributor.googleauthor | Sung Jae Shin | - |
dc.contributor.googleauthor | Eui-Cheol Shin | - |
dc.identifier.doi | 10.7774/cevr.2014.3.2.235 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02113 | - |
dc.relation.journalcode | J00573 | - |
dc.identifier.pmid | 25003098 | - |
dc.subject.keyword | DNA vaccines | - |
dc.subject.keyword | Polyfunctional T cells | - |
dc.subject.keyword | Resuscitation-promoting factor B | - |
dc.subject.keyword | Tuberculosis | - |
dc.contributor.alternativeName | Shin, Sung Jae | - |
dc.contributor.affiliatedAuthor | Shin, Sung Jae | - |
dc.citation.volume | 3 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 235 | - |
dc.citation.endPage | 243 | - |
dc.identifier.bibliographicCitation | Clinical Experimental Vaccine Research, Vol.3(2) : 235-243, 2014 | - |
dc.identifier.rimsid | 53878 | - |
dc.type.rims | ART | - |
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