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Tracheal reconstruction using chondrocytes seeded on a poly(l-lactic-co-glycolic acid)-fibrin/hyaluronan.

 Hyun Jun Hong  ;  Jae Won Chang  ;  Ju-Kyeong Park  ;  Jae Won Choi  ;  Yoo Suk Kim  ;  Yoo Seob Shin  ;  Chul-Ho Kim  ;  Eun Chang Choi 
 Journal of Biomedical Materials Research Part A, Vol.102(11) : 4142-4150, 2014 
Journal Title
 Journal of Biomedical Materials Research Part A 
Issue Date
Allografts ; Animals ; Cells, Cultured ; Chondrocytes*/metabolism ; Chondrocytes*/transplantation ; Fibrin/pharmacology* ; Hyaluronic Acid/pharmacology* ; Lactic Acid/pharmacology* ; Polyglycolic Acid/pharmacology* ; Rabbits ; Regeneration ; Respiratory Mucosa/cytology ; Respiratory Mucosa/metabolism ; Tissue Scaffolds* ; Trachea/surgery*
chondrocyte ; fibrin ; hyaluronan ; poly(l-lactic-co-glycolic acid) ; tracheal regeneration
Reconstruction of trachea is still a clinical dilemma. Tissue engineering is a recent and promising concept to resolve this problem. This study evaluated the feasibility of allogeneic chondrocytes cultured with fibrin/hyaluronic acid (HA) hydrogel and degradable porous poly(l-lactic-co-glycolic acid) (PLGA) scaffold for partial tracheal reconstruction. Chondrocytes from rabbit articular cartilage were expanded and cultured with fibrin/HA hydrogel and injected into a 5 × 10 mm-sized, curved patch-shape PLGA scaffold. After 4 weeks in vitro culture, the scaffold was implanted on a tracheal defect in eight rabbits. Six and 10 weeks postoperatively, the implanted sites were evaluated by bronchoscope and radiologic and histologic analyses. Ciliary beat frequency (CBF) of regenerated epithelium was also evaluated. None of the eight rabbits showed any sign of respiratory distress. Bronchoscopic examination did not reveal stenosis of the reconstructed trachea and the defects were completely recovered with respiratory epithelium. Computed tomography scan showed good luminal contour of trachea. Histologic data showed that the implanted chondrocytes successfully formed neocartilage with minimal granulation tissue. CBF of regenerated epithelium was similar to that of normal epithelium. Partial tracheal defect was successfully reconstructed anatomically and functionally using allogeneic chondrocytes cultured with PLGA-fibrin/HA composite scaffold.
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1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Choi, Eun Chang(최은창)
Hong, Hyun Jun(홍현준)
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