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Herniated Intervertebral Disk Induces Hypertrophy and Ossification of Ligamentum Flavum

 Young-Mi Kang  ;  Kyung-Soo Suk  ;  Byung Ho Lee  ;  Hak-Sun Kim  ;  Kwang-Il Lee  ;  Si-Young Park  ;  Hwan-Mo Lee  ;  Seong-Hwan Moon 
 Journal of Spinal Disorders & Techniques, Vol.27(7) : 382-389, 2014 
Journal Title
 Journal of Spinal Disorders & Techniques 
Issue Date
STUDY DESIGN: In vitro experiment using degenerated human ligamentum flavum (LF) and herniated intervertebral disk (IVD). OBJECTIVES: To investigate the role and effect of degenerated and herniated IVDs on LF hypertrophy and ossification. SUMMARY OF BACKGROUND DATA: Spinal stenosis is caused, in part, by hypertrophy and ossification of the LF, which are induced by aging and degenerative process. It is well known that degenerated IVDs spontaneously produce inflammatory cytokines. Therefore, we hypothesized that degenerated IVD may affect adjacent LF through secreted inflammatory cytokines. METHODS: LF and herniated lumbar IVD tissues were obtained during surgical spinal procedures. LF fibroblasts were isolated by enzymatic digestion of LF tissue. LF cell cultures were treated with disk supernatant from herniated IVDs. Secreted cytokines from IVD tissue culture were detected by enzyme-linked immunosorbent assay. After analysis of cytotoxicity, DNA synthesis was measured. Reverse transcription-polymerase chain reaction for mRNA expressions of types I, II, III, V, and XI collagen and osteocalcin, and histochemical stains were performed. RESULTS: Supernatant from tissue culture of herniated IVD showed increased production of interleukin-1α, interleukin-6, tumor necrosis factor-α, prostaglandin E2, and nitric oxide compared with disk tissue culture from traumatic condition. There was no cytotoxicity in LF cells treated with disk supernatant from herniated IVDs. There was significant increase in DNA synthesis, upregulation in mRNA expression of types III, XI collagen and osteocalcin, whereas variable expression pattern of type I and V, and strong positive stains for Von Kossa and alkaline phosphatase in LF cultures with disk supernatant. CONCLUSIONS: Degenerated and herniated IVDs provide an important pathomechanism in hypertrophy and ossification of the LF through inflammatory cytokines.
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1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
강영미(Kang, Young Mi) ORCID logo https://orcid.org/0000-0002-7686-6316
김학선(Kim, Hak Sun) ORCID logo https://orcid.org/0000-0002-8330-4688
문성환(Moon, Seong Hwan)
석경수(Suk, Kyung Soo) ORCID logo https://orcid.org/0000-0003-0633-2658
이광일(Lee, Kwang Il)
이병호(Lee, Byung Ho) ORCID logo https://orcid.org/0000-0001-7235-4981
이환모(Lee, Hwan Mo) ORCID logo https://orcid.org/0000-0002-5405-3832
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