Cited 19 times in
Homer2 Protein Regulates Plasma Membrane Ca2+ ATPase -mediated Ca2+ Signaling in Mouse Parotid Gland Acinar Cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박순홍 | - |
dc.contributor.author | 신동민 | - |
dc.contributor.author | 양유미 | - |
dc.contributor.author | 이지애 | - |
dc.contributor.author | 장인익 | - |
dc.date.accessioned | 2015-01-06T17:17:36Z | - |
dc.date.available | 2015-01-06T17:17:36Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/99704 | - |
dc.description.abstract | Homer proteins are scaffold molecules with a domain structure consisting of an N-terminal Ena/VASP homology 1 protein-binding domain and a C-terminal leucine zipper/coiled-coil domain. The Ena/VASP homology 1 domain recognizes proline-rich motifs and binds multiple Ca(2+)-signaling proteins, including G protein-coupled receptors, inositol 1,4,5-triphosphate receptors, ryanodine receptors, and transient receptor potential channels. However, their role in Ca(2+) signaling in nonexcitable cells is not well understood. In this study, we investigated the role of Homer2 on Ca(2+) signaling in parotid gland acinar cells using Homer2-deficient (Homer2(-/-)) mice. Homer2 is localized at the apical pole in acinar cells. Deletion of Homer2 did not affect inositol 1,4,5-triphosphate receptor localization or channel activity and did not affect the expression and activity of sarco/endoplasmic reticulum Ca(2+)-ATPase pumps. In contrast, Homer2 deletion markedly increased expression of plasma membrane Ca(2+)-ATPase (PMCA) pumps, in particular PMCA4, at the apical pole. Accordingly, Homer2 deficiency increased Ca(2+) extrusion by acinar cells. These findings were supported by co-immunoprecipitation of Homer2 and PMCA in wild-type parotid cells and transfected human embryonic kidney 293 (HEK293) cells. We identified a Homer-binding PPXXF-like motif in the N terminus of PMCA that is required for interaction with Homer2. Mutation of the PPXXF-like motif did not affect the interaction of PMCA with Homer1 but inhibited its interaction with Homer2 and increased Ca(2+) clearance by PMCA. These findings reveal an important regulation of PMCA by Homer2 that has a central role on PMCA-mediated Ca(2+) signaling in parotid acinar cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 24971~24979 | - |
dc.relation.isPartOf | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Acinar Cells/metabolism* | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Binding Sites/genetics | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Calcium/metabolism* | - |
dc.subject.MESH | Calcium Signaling | - |
dc.subject.MESH | Carrier Proteins/genetics | - |
dc.subject.MESH | Carrier Proteins/metabolism* | - |
dc.subject.MESH | HEK293 Cells | - |
dc.subject.MESH | Homer Scaffolding Proteins | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Inositol 1,4,5-Trisphosphate Receptors/metabolism | - |
dc.subject.MESH | Mice, Knockout | - |
dc.subject.MESH | Microscopy, Confocal | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Parotid Gland/cytology | - |
dc.subject.MESH | Parotid Gland/metabolism* | - |
dc.subject.MESH | Plasma Membrane Calcium-Transporting ATPases/genetics | - |
dc.subject.MESH | Plasma Membrane Calcium-Transporting ATPases/metabolism* | - |
dc.subject.MESH | Protein Binding | - |
dc.subject.MESH | Sarcoplasmic Reticulum Calcium-Transporting ATPases/metabolism | - |
dc.subject.MESH | Sequence Homology, Amino Acid | - |
dc.title | Homer2 Protein Regulates Plasma Membrane Ca2+ ATPase -mediated Ca2+ Signaling in Mouse Parotid Gland Acinar Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | Yu-Mi Yang | - |
dc.contributor.googleauthor | Jiae Lee | - |
dc.contributor.googleauthor | Hae Jo | - |
dc.contributor.googleauthor | Soonhong Park | - |
dc.contributor.googleauthor | Inik Chang | - |
dc.contributor.googleauthor | Shmuel Muallem | - |
dc.contributor.googleauthor | Dong Min Shin | - |
dc.identifier.doi | 10.1074/jbc.M114.577221 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01547 | - |
dc.contributor.localId | A02091 | - |
dc.contributor.localId | A03192 | - |
dc.contributor.localId | A03461 | - |
dc.contributor.localId | A05148 | - |
dc.relation.journalcode | J01258 | - |
dc.identifier.eissn | 1083-351X | - |
dc.identifier.pmid | 25049230 | - |
dc.identifier.url | http://www.jbc.org/content/289/36/24971.long | - |
dc.subject.keyword | Calcium ATPase | - |
dc.subject.keyword | Calcium Transport | - |
dc.subject.keyword | Cell Signaling | - |
dc.subject.keyword | Homer Proteins | - |
dc.subject.keyword | Parotid Gland | - |
dc.subject.keyword | Plasma Membrane Ca2+-ATPase | - |
dc.subject.keyword | Proline-rich Motif | - |
dc.subject.keyword | Protein-Protein Interaction | - |
dc.subject.keyword | Scaffold Protein | - |
dc.contributor.alternativeName | Park, Soon Hong | - |
dc.contributor.alternativeName | Shin, Dong Min | - |
dc.contributor.alternativeName | Yang, Yu-Mi | - |
dc.contributor.alternativeName | Lee, Ji Ae | - |
dc.contributor.alternativeName | Chang, In Ik | - |
dc.contributor.affiliatedAuthor | Park, Soon Hong | - |
dc.contributor.affiliatedAuthor | Shin, Dong Min | - |
dc.contributor.affiliatedAuthor | Lee, Ji Ae | - |
dc.contributor.affiliatedAuthor | Chang, In Ik | - |
dc.contributor.affiliatedAuthor | Yang, Yu Mi | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 289 | - |
dc.citation.number | 36 | - |
dc.citation.startPage | 24971 | - |
dc.citation.endPage | 24979 | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, Vol.289(36) : 24971-24979, 2014 | - |
dc.identifier.rimsid | 57069 | - |
dc.type.rims | ART | - |
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