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Real-Time Discrimination between Proliferation and Neuronal and Astroglial Differentiation of Human Neural Stem Cells

 Rimi Lee  ;  Il-Sun Kim  ;  Nalae Han  ;  Seokhwan Yun  ;  Kook In Park  ;  Kyung-Hwa Yoo 
 SCIENTIFIC REPORTS, Vol.4 : 6319, 2014 
Journal Title
Issue Date
Astrocytes/cytology* ; Brain/cytology* ; Cell Proliferation/physiology ; Cells, Cultured ; Electric Capacitance ; Electric Stimulation ; Humans ; Neural Stem Cells/cytology* ; Neurogenesis/physiology* ; Neurons/cytology*
Neural stem cells (NSCs) are characterized by a capacity for self-renewal, differentiation into multiple neural lineages, all of which are considered to be promising components for neural regeneration. However, for cell-replacement therapies, it is essential to monitor the process of in vitro NSC differentiation and identify differentiated cell phenotypes. We report a real-time and label-free method that uses a capacitance sensor array to monitor the differentiation of human fetal brain-derived NSCs (hNSCs) and to identify the fates of differentiated cells. When hNSCs were placed under proliferation or differentiation conditions in five media, proliferating and differentiating hNSCs exhibited different frequency and time dependences of capacitance, indicating that the proliferation and differentiation status of hNSCs may be discriminated in real-time using our capacitance sensor. In addition, comparison between real-time capacitance and time-lapse optical images revealed that neuronal and astroglial differentiation of hNSCs may be identified in real-time without cell labeling.
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1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pediatrics (소아청소년과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Il-Sun(김일선) ORCID logo https://orcid.org/0000-0003-4033-4323
Park, Kook In(박국인) ORCID logo https://orcid.org/0000-0001-8499-9293
Yun, Seok Hwan(윤석환)
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