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Optimal administration routes for adipose-derived stem cells therapy in ischaemic flaps

Authors
 Dong Won Lee  ;  Yeo Reum Jeon  ;  Eul Je Cho  ;  Jong Hwa Kang  ;  Dae Hyun Lew 
Citation
 JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, Vol.8(8) : 596-603, 2014 
Journal Title
 JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE 
ISSN
 1932-6254 
Issue Date
2014
MeSH
Adipose Tissue/cytology* ; Animals ; Capillaries/pathology ; Drug Administration Routes ; Female ; Flow Cytometry ; Humans ; Immunohistochemistry ; Ischemia/therapy* ; Laser-Doppler Flowmetry ; Necrosis ; Neovascularization, Physiologic ; Organic Chemicals/metabolism ; Platelet Endothelial Cell Adhesion Molecule-1/metabolism ; Rats, Sprague-Dawley ; Skin/blood supply ; Stem Cell Transplantation* ; Surgical Flaps/blood supply*
Keywords
adipose-derived stem cell ; administration route ; angiogenesis ; collagen sponge ; distribution ; flap survival
Abstract
Improvement of flap survival represents an ongoing challenge in reconstructive surgery. The angiogenic potential of adipose-derived stem cells (ASCs) offers a promising approach to improve the viability of random pattern flaps. Recently, to maximize the therapeutic effects of ASCs, increasing focus is being placed on how to deliver the stem cells to target lesions. The purpose of the present study was to compare the effectiveness of different administration routes of ASCs to improve the viability of the random pattern skin flap. ASCs labelled with PKH26 were applied via four methods to the cranially-based random pattern skin flaps of rats: (a) intravenous injection; (b) subcutaneous injection; (c) application with collagen sponge seeding; and (d) application with fibrin glue seeding. ASCs led to a significant increase in flap viability in the subcutaneous injection group and the collagen sponge group. Cutaneous blood flow was increased in the intravenous injection, subcutaneous injection and collagen sponge groups. Capillary density in the intravenous injection group and collagen sponge group was significantly greater than in the control group (no treatment). PKH26-positive cells via the collagen sponge were distributed more densely within the flap than in other groups. This study demonstrated that the collagen sponge method delivered ASCs most effectively within the flap and increased flap vascularity. The clinical therapeutic effects of ASCs can therefore be maximized when the optimal delivery route is chosen.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/term.1552/abstract
DOI
10.1002/term.1552
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
Yonsei Authors
Lew, Dae Hyun(유대현)
Lee, Dong Won(이동원) ORCID logo https://orcid.org/0000-0003-0046-3139
Jeon, Yeo Reum(전여름)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/99608
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