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Retroviral Expression of Human Arginine Decarboxylase Reduces Oxidative Stress Injury in Mouse Cortical Astrocytes.

DC FieldValueLanguage
dc.contributor.author박경아-
dc.contributor.author이원택-
dc.contributor.author이종은-
dc.contributor.author홍사민-
dc.date.accessioned2015-01-06T17:09:41Z-
dc.date.available2015-01-06T17:09:41Z-
dc.date.issued2014-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/99449-
dc.description.abstractBACKGROUND: In physiologic and pathologic conditions of the central nervous system (CNS), astrocytes are a double-edged sword. They not only support neuronal homeostasis but also contribute to increases in neuronal demise. A large body of experimental evidence has shown that impaired astrocytes play crucial roles in the pathologic process of cerebral ischemia; therefore, astrocytes may represent a breakthrough target for neuroprotective therapeutic strategies. Agmatine, an endogenous polyamine catalyzed from L-arginine by arginine decarboxylase (ADC), is a neuromodulator and it protects neurons/glia against various injuries. RESULTS: In this investigation, agmatine-producing mouse cortical astrocytes were developed through transduction of the human ADC gene. Cells were exposed to oxygen-glucose deprivation (OGD) and restored to a normoxic glucose-supplied condition. Intracellular levels of agmatine were measured by high performance liquid chromatography. Cell viability was evaluated by Hoechest/propidium iodide nuclear staining and lactate dehydrogenase assay. Expression of inducible nitric oxide synthase (iNOS) and matrix metalloproteinase s (MMPs) were assessed by a reverse transcription polymerase chain reaction, Western immunoblots, and immunofluorescence. We confirmed that ADC gene-expressed astrocytes produce a great amount of agmatine. These cells were highly resistant to not only OGD but also restoration, which mimicked ischemia-reperfusion injury in vivo. The neuroprotective effects of ADC seemed to be related to its ability to attenuate expression of iNOS and MMPs. CONCLUSION: Our findings imply that astrocytes can be reinforced against oxidative stress by endogenous agmatine production through ADC gene transduction. The results of this study provide new insights that may lead to novel therapeutic approaches to reduce cerebral ischemic injuries.-
dc.description.statementOfResponsibilityopen-
dc.format.extent1~10-
dc.relation.isPartOfBMC NEUROSCIENCE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAgmatine/metabolism*-
dc.subject.MESHAnimals-
dc.subject.MESHAstrocytes/metabolism*-
dc.subject.MESHCarboxy-Lyases/genetics*-
dc.subject.MESHCarboxy-Lyases/metabolism*-
dc.subject.MESHCell Hypoxia*-
dc.subject.MESHCell Survival-
dc.subject.MESHCells, Cultured-
dc.subject.MESHCerebral Cortex/cytology*-
dc.subject.MESHCerebral Cortex/physiology-
dc.subject.MESHGenetic Vectors-
dc.subject.MESHGlucose/deficiency*-
dc.subject.MESHHumans-
dc.subject.MESHIntracellular Space/metabolism-
dc.subject.MESHMatrix Metalloproteinases/metabolism-
dc.subject.MESHMice-
dc.subject.MESHNitric Oxide Synthase Type II/metabolism-
dc.subject.MESHOxidative Stress*-
dc.subject.MESHRetroviridae/genetics-
dc.titleRetroviral Expression of Human Arginine Decarboxylase Reduces Oxidative Stress Injury in Mouse Cortical Astrocytes.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Anatomy (해부학)-
dc.contributor.googleauthorSamin Hong-
dc.contributor.googleauthorMi Ran Son-
dc.contributor.googleauthorKyungeun Yun-
dc.contributor.googleauthorWon Taek Lee-
dc.contributor.googleauthorKyung Ah Park-
dc.contributor.googleauthorJong Eun Lee-
dc.identifier.doi10.1186/1471-2202-15-99-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01424-
dc.contributor.localIdA03007-
dc.contributor.localIdA04395-
dc.contributor.localIdA03146-
dc.relation.journalcodeJ00369-
dc.identifier.eissn1471-2202-
dc.identifier.pmid25156824-
dc.subject.keywordAgmatine-
dc.subject.keywordArginine decarboxylase-
dc.subject.keywordAstrocyte-
dc.subject.keywordNeuroprotection-
dc.subject.keywordOxidative stress-
dc.contributor.alternativeNamePark, Kyung Ah-
dc.contributor.alternativeNameLee, Won Taek-
dc.contributor.alternativeNameLee, Jong Eun-
dc.contributor.alternativeNameHong, Sa Min-
dc.contributor.affiliatedAuthorPark, Kyung Ah-
dc.contributor.affiliatedAuthorLee, Won Taek-
dc.contributor.affiliatedAuthorHong, Sa Min-
dc.contributor.affiliatedAuthorLee, Jong Eun-
dc.citation.volume15-
dc.citation.number99-
dc.citation.startPage1-
dc.citation.endPage10-
dc.identifier.bibliographicCitationBMC NEUROSCIENCE, Vol.15(99) : 1-10, 2014-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers

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