MDVP and Praat are measured by nine variables in common; F0, jitter local, jitter absolute, jitter relative average perturbation, jitter period perturbation quotient, shimmer local, shimmer dB, shimmer amplitude perturbation quotient, and NHR. In the present study, 30 female subjects were divided by their disorders(control group, vocal nodule group), ages(from 18 to 50 years old), gender(women), and severities of voice disorder(GRBAS-G0, G1, G2). Then, the subjects' vowel /a/ was evaluated by MDVP and Praat. First, jitter and shimmer variables of the MDVP were significantly different by severities. Praat showed different jitter, shimmer, and NHR parameters by severities. Second, jitter and NHR levels of MDVP were meaningfully higher than Praat regardless their severities. The result of the research confirms the relationships among GRBAS, MDVP and Praat as well as the differences in acoustic variables between MDVP and Praat.