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MicroRNA-495 Inhibits chondrogenic differentiation in human mesenchymal stem cells by targeting Sox9

DC Field Value Language
dc.contributor.author백승일-
dc.contributor.author윤동석-
dc.contributor.author이경미-
dc.contributor.author이슬기-
dc.contributor.author이진우-
dc.contributor.author장연수-
dc.date.accessioned2015-01-06T17:07:10Z-
dc.date.available2015-01-06T17:07:10Z-
dc.date.issued2014-
dc.identifier.issn1547-3287-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/99377-
dc.description.abstractThe chondrogenic differentiation process of human mesenchymal stem cells (hMSCs) passes through multiple stages, which are carried out by various factors and their interactions. Recently, microRNAs that regulate chondrogenic differentiation have been reported. However, microRNA that regulates SRY-related high mobility group-box gene 9 (Sox9), a chondrogenic key factor, has not been identified in hMSC. In this study, we identified that microRNA-495 (miR-495) is an important regulator of hMSC chondrogenic differentiation. In our microarray, miR-495 was downregulated during transforming growth factor (TGF)-β3-induced chondrogenic differentiation of hMSCs in vitro. We found that there is an miR-495 binding site in the 3′ untranslated region (3′UTR) of Sox9. We confirmed opposite expression between miR-495 and Sox9 by using real-time polymerase chain reaction. Further, overexpression of miR-495 inhibited Sox9 expression, and repression of miR-495 increased expression of Sox9 in SW1353 cells and hMSCs. Additionally, luciferase analysis revealed that miR-495 directly binds to the Sox9 3′UTR, and we confirmed a seed sequence of miR-495 on the Sox9 3′UTR. Subsequently, overexpression of miR-495 repressed the expression of the extracellular matrix (ECM) protein, such as type II collagen (Col2A1), aggrecan, and proteoglycan products, whereas inhibition of miR-495 increased their expression. Collectively, this study indicates that miR-495 directly targets Sox9, ultimately leading to the regulation of chondrogenic differentiation in hMSCs.-
dc.description.statementOfResponsibilityopen-
dc.format.extent1798~1808-
dc.relation.isPartOfSTEM CELLS AND DEVELOPMENT-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESH3' Untranslated Regions/genetics-
dc.subject.MESHAdult-
dc.subject.MESHBase Sequence-
dc.subject.MESHBiomarkers/metabolism-
dc.subject.MESHCell Differentiation/drug effects-
dc.subject.MESHCell Differentiation/genetics*-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHCell Lineage/drug effects-
dc.subject.MESHCell Lineage/genetics-
dc.subject.MESHCells, Cultured-
dc.subject.MESHChondrogenesis/drug effects-
dc.subject.MESHChondrogenesis/genetics*-
dc.subject.MESHGene Expression Regulation/drug effects-
dc.subject.MESHGlycosaminoglycans/metabolism-
dc.subject.MESHHumans-
dc.subject.MESHImmunohistochemistry-
dc.subject.MESHMesenchymal Stromal Cells/cytology*-
dc.subject.MESHMesenchymal Stromal Cells/drug effects-
dc.subject.MESHMesenchymal Stromal Cells/metabolism-
dc.subject.MESHMicroRNAs/genetics-
dc.subject.MESHMicroRNAs/metabolism*-
dc.subject.MESHMiddle Aged-
dc.subject.MESHMolecular Sequence Data-
dc.subject.MESHProtein Binding/drug effects-
dc.subject.MESHProtein Binding/genetics-
dc.subject.MESHProteoglycans/metabolism-
dc.subject.MESHRNA, Messenger/genetics-
dc.subject.MESHRNA, Messenger/metabolism-
dc.subject.MESHSOX9 Transcription Factor/genetics*-
dc.subject.MESHSOX9 Transcription Factor/metabolism-
dc.subject.MESHTransforming Growth Factor beta3/pharmacology-
dc.subject.MESHYoung Adult-
dc.titleMicroRNA-495 Inhibits chondrogenic differentiation in human mesenchymal stem cells by targeting Sox9-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Orthopedic Surgery (정형외과학)-
dc.contributor.googleauthorLee Seulgi-
dc.contributor.googleauthorYoon Dong Suk-
dc.contributor.googleauthorPaik Seungil-
dc.contributor.googleauthorLee Kyoung-Mi-
dc.contributor.googleauthorJang Yeonsue-
dc.contributor.googleauthorLee Jin Woo-
dc.identifier.doi10.1089/scd.2013.0609-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02909-
dc.contributor.localIdA01825-
dc.contributor.localIdA02547-
dc.contributor.localIdA04619-
dc.contributor.localIdA03230-
dc.contributor.localIdA03449-
dc.relation.journalcodeJ02684-
dc.identifier.eissn1557-8534-
dc.identifier.pmid24654627-
dc.identifier.urlhttp://online.liebertpub.com/doi/abs/10.1089/scd.2013.0609-
dc.contributor.alternativeNamePaik, Seung Il-
dc.contributor.alternativeNameYoon, Dong Suk-
dc.contributor.alternativeNameLee, Kyung Mi-
dc.contributor.alternativeNameLee, Seul Gi-
dc.contributor.alternativeNameLee, Jin Woo-
dc.contributor.alternativeNameJang, Yeon Sue-
dc.contributor.affiliatedAuthorLee, Seul Gi-
dc.contributor.affiliatedAuthorPaik, Seung Il-
dc.contributor.affiliatedAuthorYoon, Dong Suk-
dc.contributor.affiliatedAuthorLee, Kyoung Mi-
dc.contributor.affiliatedAuthorLee, Jin Woo-
dc.contributor.affiliatedAuthorJang, Yeon Sue-
dc.rights.accessRightsfree-
dc.citation.volume23-
dc.citation.number15-
dc.citation.startPage1798-
dc.citation.endPage1808-
dc.identifier.bibliographicCitationSTEM CELLS AND DEVELOPMENT, Vol.23(15) : 1798-1808, 2014-
dc.identifier.rimsid57225-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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