Cited 7 times in
Pre-S mutations of hepatitis B virus affect genome replication and expression of surface antigens
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김승택 | - |
dc.contributor.author | 김현숙 | - |
dc.contributor.author | 박전한 | - |
dc.contributor.author | 안상훈 | - |
dc.contributor.author | 이희승 | - |
dc.contributor.author | 정애리 | - |
dc.contributor.author | 최성훈 | - |
dc.contributor.author | 한광협 | - |
dc.contributor.author | 김경식 | - |
dc.contributor.author | 김범경 | - |
dc.date.accessioned | 2015-01-06T16:52:21Z | - |
dc.date.available | 2015-01-06T16:52:21Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0815-9319 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/98932 | - |
dc.description.abstract | BACKGROUNDS AND AIMS: In chronic hepatitis B virus (HBV) infection, quantitative HBV surface antigen (qHBsAg) is useful for monitoring viral replication and treatment responses. We aimed to determine whether pre-S mutations have any effect on circulating qHBsAg. METHODS: Plasmids expressing 1–8 amino acid deletion in pre-S1 ("pre-S1Δ1-8") and 3-25 amino acid deletion in pre-S2 ("pre-S2Δ3-25") were constructed. At 72 h posttransfection into Huh7 cells, qHBsAg were measured using electrochemiluminescence immunoassay analyzer. To mimic milieus of quasispecies, we co-transfected either pre-S1Δ1-8 or pre-S2Δ3-25 with wild type (WT). RESULTS: Pre-S mutations affected transcription and replication ability of HBV because of altered overlapping polymerase. Compared with WT, extracellular qHBsAg in pre-S1Δ1-8 and pre-S2Δ3-25 were on average 3.87-fold higher and 0.92-fold lower, respectively, whereas intracellular qHBsAg in pre-S1Δ1-8 and pre-S2Δ3-25 were 0.57-fold lower and 1.60-fold higher, respectively. Immunofluorescence staining of cellular HBsAg showed that pre-S1Δ1-8 had less staining and that pre-S2Δ3-25 had denser staining. As ratios of either pre-S1Δ1-8 or pre-S2Δ3-25:WT increased from 0:10 to 10:0 gradually, relative extracellular qHBsAg increased from 1.0 to 3.85 in pre-S1Δ1-8 co-transfection, whereas those decreased from 1.0 to 0.88 in pre-S2Δ3-25 co-transfection. CONCLUSION: Pre-S mutations exhibit different phenotypes of genome replication and HBsAg expression according to their locations. Thus, qHBsAg level for diagnosis and prognostification in chronic HBV infection should be used more cautiously, considering emergences of pre-S deletion mutants. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 843~850 | - |
dc.relation.isPartOf | JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Pre-S mutations of hepatitis B virus affect genome replication and expression of surface antigens | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Beom Kyung Kim | - |
dc.contributor.googleauthor | Sung Hoon Choi | - |
dc.contributor.googleauthor | Sung Hyun Ahn | - |
dc.contributor.googleauthor | Ae Ri Chung | - |
dc.contributor.googleauthor | Yong Kwang Park | - |
dc.contributor.googleauthor | Kwang-Hyub Han | - |
dc.contributor.googleauthor | Seungtaek Kim | - |
dc.contributor.googleauthor | Hyon-Suk Kim | - |
dc.contributor.googleauthor | Jeon Han Park | - |
dc.contributor.googleauthor | Kyung Sik Kim | - |
dc.contributor.googleauthor | Hee Seung Lee | - |
dc.contributor.googleauthor | Yong Sang Cho | - |
dc.contributor.googleauthor | Kyun-Hwan Kim | - |
dc.contributor.googleauthor | Sang Hoon Ahn | - |
dc.identifier.doi | 10.1111/jgh.12415 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00661 | - |
dc.contributor.localId | A01641 | - |
dc.contributor.localId | A02226 | - |
dc.contributor.localId | A03349 | - |
dc.contributor.localId | A03649 | - |
dc.contributor.localId | A04268 | - |
dc.contributor.localId | A00299 | - |
dc.contributor.localId | A00487 | - |
dc.contributor.localId | A04086 | - |
dc.contributor.localId | A01117 | - |
dc.relation.journalcode | J01417 | - |
dc.identifier.eissn | 1440-1746 | - |
dc.identifier.pmid | 24843434 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/jgh.12415/abstract | - |
dc.subject.keyword | Edema | - |
dc.subject.keyword | Pioglitazone | - |
dc.contributor.alternativeName | Kim, Seung Taek | - |
dc.contributor.alternativeName | Kim, Hyon Suk | - |
dc.contributor.alternativeName | Park, Jeon Han | - |
dc.contributor.alternativeName | Ahn, Sang Hoon | - |
dc.contributor.alternativeName | Lee, Hee Seung | - |
dc.contributor.alternativeName | Chung, Ae Ri | - |
dc.contributor.alternativeName | Choi, Sung Hoon | - |
dc.contributor.alternativeName | Han, Kwang Hyup | - |
dc.contributor.alternativeName | Kim, Kyung Sik | - |
dc.contributor.alternativeName | Kim, Beom Kyung | - |
dc.contributor.affiliatedAuthor | Kim, Seung Taek | - |
dc.contributor.affiliatedAuthor | Park, Jeon Han | - |
dc.contributor.affiliatedAuthor | Ahn, Sang Hoon | - |
dc.contributor.affiliatedAuthor | Lee, Hee Seung | - |
dc.contributor.affiliatedAuthor | Chung, Ae Ri | - |
dc.contributor.affiliatedAuthor | Han, Kwang Hyup | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Sik | - |
dc.contributor.affiliatedAuthor | Kim, Beom Kyung | - |
dc.contributor.affiliatedAuthor | Choi, Sung Hoon | - |
dc.contributor.affiliatedAuthor | Kim, Hyon Suk | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 29 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 843 | - |
dc.citation.endPage | 850 | - |
dc.identifier.bibliographicCitation | JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, Vol.29(4) : 843-850, 2014 | - |
dc.identifier.rimsid | 54292 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.