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G0/G1 switch gene 2 has a critical role in adipocyte differentiation

Authors
 H Choi  ;  H Lee  ;  T-H Kim  ;  H J Kim  ;  Y J Lee  ;  S J Lee  ;  J H Yu  ;  D Kim  ;  K-S Kim  ;  S W Park  ;  J-w Kim 
Citation
 Cell Death and Differentiation, Vol.21(7) : 1071-1080, 2014 
Journal Title
 Cell Death and Differentiation 
ISSN
 1350-9047 
Issue Date
2014
Abstract
Mouse 3T3-L1 preadipocytes differentiate into adipocytes when treated with 3-isobutyl-1-methylxanthine, dexamethasone, and insulin. Although mechanisms of adipogenesis, including transcriptional cascades, are understood, it is still unclear how clonally expanded cells eventually enter the terminal differentiation program. From gene expression profile studies, we identified G0/G1 switch gene 2 (G0s2) as a novel regulator of adipogenesis. The gene was found to be expressed at a higher level in white and brown adipose tissues, and it was induced in 3T3-L1 cells by hormonal treatment. Importantly, G0s2 expression was closely associated with the transition from mitotic clonal expansion to terminal differentiation. Knockdown of G0s2 expression with siRNA inhibited adipocyte differentiation, whereas constitutive overexpression of G0s2 accelerated differentiation of preadipocytes to mature adipocytes. Expression of G0s2 was found to be regulated by peroxisome proliferator-activated receptor γ (PPARγ), which is a well-known regulator of adipocyte differentiation. Absence of either PPARγ or G0s2 expression resulted in apoptotic pathway activation before terminal differentiation. To determine whether G0s2 has a role in vivo, G0s2-knockout mice were generated. The knockout mice were normal in appearance, but they had less adipose mass than wild-type littermates. Mouse embryonic fibroblast cells from G0s2-deficient mice exhibited impaired adipogenesis and contained unusually small intracellular lipid droplets, suggesting that G0s2 has a role in lipid droplet formation. Our studies demonstrate that G0s2 has an important role in adipogenesis and accumulation of triacylglycerol.
Full Text
http://www.nature.com/cdd/journal/v21/n7/full/cdd201426a.html
DOI
10.1038/cdd.2014.26
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
김경섭(Kim, Kyung Sup) ORCID logo https://orcid.org/0000-0001-8483-8537
김다함(Kim, Daham) ORCID logo https://orcid.org/0000-0003-1871-686X
김재우(Kim, Jae Woo) ORCID logo https://orcid.org/0000-0001-5456-9495
김태현(Kim, Tae Hyun)
김효정(Kim, Hyo Jung) ORCID logo https://orcid.org/0000-0002-3514-1247
박상욱(Park, Sahng Wook) ORCID logo https://orcid.org/0000-0002-9594-7074
최현진(Choi, Hyeon Jin)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/98822
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