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G0/G1 switch gene 2 has a critical role in adipocyte differentiation

 H Choi  ;  H Lee  ;  T-H Kim  ;  H J Kim  ;  Y J Lee  ;  S J Lee  ;  J H Yu  ;  D Kim  ;  K-S Kim  ;  S W Park  ;  J-w Kim 
 CELL DEATH AND DIFFERENTIATION, Vol.21(7) : 1071-1080, 2014 
Journal Title
Issue Date
3T3-L1 Cells ; Adipocytes/physiology* ; Adipogenesis* ; Adiposity ; Animals ; Apoptosis ; Cell Cycle Proteins/physiology* ; Genes, Switch ; Lipase/metabolism ; Lipid Droplets/metabolism ; Lipid Metabolism ; Mice ; Mice, Knockout ; Obesity/metabolism ; PPAR gamma/metabolism ; Triglycerides/metabolism
Mouse 3T3-L1 preadipocytes differentiate into adipocytes when treated with 3-isobutyl-1-methylxanthine, dexamethasone, and insulin. Although mechanisms of adipogenesis, including transcriptional cascades, are understood, it is still unclear how clonally expanded cells eventually enter the terminal differentiation program. From gene expression profile studies, we identified G0/G1 switch gene 2 (G0s2) as a novel regulator of adipogenesis. The gene was found to be expressed at a higher level in white and brown adipose tissues, and it was induced in 3T3-L1 cells by hormonal treatment. Importantly, G0s2 expression was closely associated with the transition from mitotic clonal expansion to terminal differentiation. Knockdown of G0s2 expression with siRNA inhibited adipocyte differentiation, whereas constitutive overexpression of G0s2 accelerated differentiation of preadipocytes to mature adipocytes. Expression of G0s2 was found to be regulated by peroxisome proliferator-activated receptor γ (PPARγ), which is a well-known regulator of adipocyte differentiation. Absence of either PPARγ or G0s2 expression resulted in apoptotic pathway activation before terminal differentiation. To determine whether G0s2 has a role in vivo, G0s2-knockout mice were generated. The knockout mice were normal in appearance, but they had less adipose mass than wild-type littermates. Mouse embryonic fibroblast cells from G0s2-deficient mice exhibited impaired adipogenesis and contained unusually small intracellular lipid droplets, suggesting that G0s2 has a role in lipid droplet formation. Our studies demonstrate that G0s2 has an important role in adipogenesis and accumulation of triacylglycerol.
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1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Sup(김경섭) ORCID logo https://orcid.org/0000-0001-8483-8537
Kim, Daham(김다함) ORCID logo https://orcid.org/0000-0003-1871-686X
Kim, Jae Woo(김재우) ORCID logo https://orcid.org/0000-0001-5456-9495
Kim, Tae Hyun(김태현)
Kim, Hyo Jung(김효정) ORCID logo https://orcid.org/0000-0002-3514-1247
Park, Sahng Wook(박상욱) ORCID logo https://orcid.org/0000-0002-9594-7074
Choi, Hyeon Jin(최현진)
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