0 185

Cited 26 times in

In vivo emergence of colistin resistance in Acinetobacter baumannii clinical isolates of sequence type 357 during colistin treatment

Authors
 Yoonjung Kim  ;  Il Kwon Bae  ;  Hyukmin Lee  ;  Seok Hoon Jeong  ;  Dongeun Yong  ;  Kyungwon Lee 
Citation
 DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, Vol.79(3) : 362-366, 2014 
Journal Title
 DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE 
ISSN
 0732-8893 
Issue Date
2014
MeSH
Acinetobacter Infections/drug therapy* ; Acinetobacter Infections/microbiology* ; Acinetobacter baumannii/classification ; Acinetobacter baumannii/drug effects* ; Acinetobacter baumannii/genetics ; Acinetobacter baumannii/isolation & purification* ; Aged ; Anti-Bacterial Agents/therapeutic use* ; Bacterial Proteins/genetics ; Colistin/therapeutic use* ; Drug Resistance, Bacterial* ; Electrophoresis, Gel, Pulsed-Field ; Female ; Genotype ; Humans ; Male ; Middle Aged ; Molecular Typing ; Mutation ; Polymorphism, Restriction Fragment Length ; Selection, Genetic ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ; Transcription Factors/genetics
Keywords
Lipid A ; Lipopolysaccharide ; Phosphoethanolamine ; PmrB gene ; Polymyxins
Abstract
This study was performed to investigate the mechanisms of in vivo acquisition of colistin resistance in A. baumannii during colistin treatment. Three colistin-susceptible/resistant pairs of A. baumannii were recovered from patients who underwent colistin treatment. All of the 6 isolates included in this study shared an identical sequence type (ST), ST375, and they showed identical SmaI-macrorestriction patterns by pulsed-field gel electrophoresis. The individual colistin-resistant isolates harbored distinct mutations in the pmrB gene. Mutations detected in the pmrB gene were Ala227Val, Pro233Ser, and frame shift from Phe26. In matrix-assisted laser desorption ionization–time of flight analysis, colistin-resistant isolates were different from their colistin-susceptible counterparts, and they showed additional distinct peaks at 1852 m/z, 1937 m/z, 1954 m/z, 1975 m/z, 2034 m/z, and 2157 m/z. In vivo selection of colistin-resistant A. baumannii occurred independently in strains of ST357 during colistin treatment, and the strains acquired colistin resistance via mutations in the pmrB gene resulting in modification of lipid A components.
Full Text
http://www.sciencedirect.com/science/article/pii/S0732889314001606
DOI
10.1016/j.diagmicrobio.2014.03.027
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/98813
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse