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The changes of PCR ribotype and antimicrobial resistance of Clostridium difficile in a tertiary care hospital over 10 years

Authors
 Jong-Han Lee  ;  Yangsoon Lee  ;  Kyungwon Lee  ;  Thomas V. Riley  ;  Heejung Kim 
Citation
 JOURNAL OF MEDICAL MICROBIOLOGY, Vol.6(63) : 819-823, 2014 
Journal Title
 JOURNAL OF MEDICAL MICROBIOLOGY 
ISSN
 0022-2615 
Issue Date
2014
MeSH
Anti-Bacterial Agents/pharmacology* ; Bacterial Toxins/genetics ; Bacterial Toxins/metabolism ; Clostridium difficile/drug effects* ; Clostridium difficile/genetics* ; DNA Gyrase/genetics ; DNA Gyrase/metabolism ; Drug Resistance, Bacterial* ; Gene Expression Regulation, Bacterial/physiology ; Humans ; Mutation ; Polymerase Chain Reaction/methods* ; Ribotyping* ; Tertiary Care Centers ; Time Factors
Abstract
The aims of this study were to investigate any change in PCR ribotypes and to determine the antimicrobial resistance of common PCR ribotypes over a 10-year period in a tertiary care hospital. We conducted PCR ribotyping, antimicrobial susceptibility testing and DNA gyrase sequencing to identify changes in 1407 Clostridium difficile non-duplicated isolates obtained between 2000 and 2009. A total of 74 different ribotypes were found. The most prevalent ribotype was ribotype 001 (26.1 %). The prevalence of ribotype 017 was 17 % and that of ribotype 014/020 was 9.6 %. Ribotyping showed that the prevalence of ribotype 001 decreased and the prevalence of ribotypes 017, 014/020 and 018 increased over the 10 years. Antimicrobial resistance rates in prevalent ribotypes were: clindamycin, 81 %; cefotetan, 19 %; moxifloxacin, 42 %; imipenem, 8 %; ciprofloxacin, 100 % and erythromycin, 80 %. Ribotype 018 showed greater antimicrobial resistance than other ribotypes. All ribotype 018 strains showing moxifloxacin resistance had a substitution of a gyrA coding amino acid (Thr82 to Ile). This study will help the understanding of PCR ribotype trends and antimicrobial resistance of C. difficile in Korea.
Full Text
http://jmm.sgmjournals.org/content/63/Pt_6/819
DOI
10.1099/jmm.0.072082-0
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Heejung(김희정) ORCID logo https://orcid.org/0000-0002-0190-703X
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Lee, Yang Soon(이양순)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/98748
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