97 195

Cited 2 times in

Effect of Hypoxic Paracrine Media on Calcium-Regulatory Proteins in Infarcted Rat Myocardium

 Byeong-Wook Song  ;  Hye Jin Hwang  ;  Minji Seung  ;  Moon-Hyoung Lee 
 Korean Circulation Journal, Vol.44(1) : 16-21, 2014 
Journal Title
 Korean Circulation Journal 
Issue Date
Calcium homeostasis endoplasmic reticulum protein ; Myocardial infarction ; Paracrine communication ; Stem cells
BACKGROUND AND OBJECTIVES: An increase in intracellular calcium concentration due to loss of Ca(2+) homeostasis triggers arrhythmia or cardiac cell death in the heart. Paracrine factors released from stem cells have beneficial cardioprotective effects. However, the mechanism of modulation of Ca(2+) homeostasis by paracrine factors in ischemic myocardium remains unclear. MATERIALS AND METHODS: We isolated rat bone marrow-derived mesenchymal stem cells (MSCs), and prepared paracrine media (PM) from MSCs under hypoxic or normoxic conditions (hypoxic PM and normoxic PM). We induced rat myocardial infarction by left anterior descending ligation for 1 hour, and treated PM into the border region of infarcted myocardium (n=6/group) to identify the alteration in calcium-regulated proteins. We isolated and stained the heart tissue with specific calcium-related antibodies after 11 days. RESULTS: The hypoxic PM treatment increased Ca(2+)-related proteins such as L-type Ca(2+) channel, sarcoplasmic reticulum Ca(2+) ATPase, Na(+)/K(+) ATPase, and calmodulin, whereas the normoxic PM treatment increased those proteins only slightly. The sodium-calcium exchanger was significantly reduced by hypoxic PM treatment, compared to moderate suppression by the normoxic PM treatment. CONCLUSION: Our results suggest that hypoxic PM was significantly associated with the positive regulation of Ca(2+) homeostasis in infarcted myocardium.
Files in This Item:
T201401001.pdf Download
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Seung, Min Ji(승민지)
Lee, Moon Hyoung(이문형) ORCID logo https://orcid.org/0000-0002-7268-0741
Hwang, Hye Jin(황혜진)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.