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The effective control of a bleeding injury using a medical adhesive containing batroxobin

 Kyung Eun You  ;  Min-Ah Koo  ;  Dae-Hyung Lee  ;  Byeong-Ju Kwon  ;  Mi Hee Lee  ;  Suong-Hyu Hyon  ;  Young Seomun  ;  Jong-Tak Kim  ;  Jong-Chul Park 
 BIOMEDICAL MATERIALS, Vol.9(2) : 25002, 2014 
Journal Title
Issue Date
Adhesives ; Aldehydes ; Animals ; Bandages* ; Batroxobin/administration & dosage* ; Blood Coagulation/drug effects* ; Bothrops ; Dextrans/chemistry ; Femoral Artery/pathology ; Fibrin/chemistry ; Fibrinogen/chemistry ; Hemorrhage/drug therapy* ; Hemostasis ; Hemostatics/administration & dosage* ; Humans ; Liver/metabolism ; Male ; Mice ; Mice, Inbred ICR ; Polylysine/chemistry ; Rats ; Rats, Sprague-Dawley ; Recombinant Proteins/chemistry
batroxobin ; hemostatic adhesive ; topical hemostatic agent ; blood coagulation ; bleeding
Many types of hemostatic agents have been studied for the effective control of bleeding. In this study, a powdery medical adhesive composed of aldehyded dextran and ε-poly (L-lysine) was used with the recombinant batroxobin. Batroxobin is a venomous component from the snake Bothrops atrox moojeni and catalyzes fibrinogen conversion to form soluble fibrin clots. This research aims to examine the performance of the batroxobin-containing adhesive for hemostasis, and evaluate its potential as a novel hemostatic adhesive. The fibrinogen conversion ability of batroxobin was evaluated by a fibrinogen clotting assay and a whole blood clotting assay. Both experiments demonstrated the effectiveness of the batroxobin-containing adhesive for blood clot formation. Animal experiments were also conducted. After a pricking wound was made in an ICR (imprinting control region) mouse liver, the adhesive and various concentrations of batroxobin were applied. The total amount of blood loss was reduced with increasing concentrations of batroxobin. For excessive bleeding conditions, the femoral artery wound model of SD (Sprague-Dawley) rats was adopted. With higher concentrations of batroxobin, hemostasis was more rapidly achieved. Histological analysis of the liver model also supports the hemostatic effects through fibrin clot formation. In conclusion, batroxobin and medical adhesive effectively facilitate blood coagulation, and could be developed for clinical use.
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1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers
Yonsei Authors
Koo, Min-Ah(구민아) ORCID logo https://orcid.org/0000-0002-8671-1131
Kwon, Byeong-Ju(권병주) ORCID logo https://orcid.org/0000-0001-9916-0546
You, Kyung Eun(류경은)
Park, Jong Chul(박종철) ORCID logo https://orcid.org/0000-0003-0083-5991
Lee, Dae Hyung(이대형)
Lee, Mi Hee(이미희) ORCID logo https://orcid.org/0000-0002-9630-7044
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