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Wogonin ameliorates hyperglycemia and dyslipidemia via PPARa activation in db/db mice

Authors
 Eun-Jung Bak  ;  Jinmoon Kim  ;  Yun Hui Choi  ;  Ji-Hye Kim  ;  Dong-Eun Lee  ;  Gye-Hyeong Woo  ;  Jeong-Heon Cha  ;  Yun-Jung Yoo 
Citation
 CLINICAL NUTRITION, Vol.33(1) : 156-163, 2014 
Journal Title
 CLINICAL NUTRITION 
ISSN
 0261-5614 
Issue Date
2014
MeSH
3T3-L1 Cells ; AMP-Activated Protein Kinases/genetics ; AMP-Activated Protein Kinases/metabolism ; Adipocytes/drug effects ; Adiponectin/blood ; Adiponectin/genetics ; Adipose Tissue, White/metabolism ; Animals ; Blood Glucose/metabolism ; Body Weight/drug effects ; Cell Differentiation/drug effects ; Cholesterol/blood ; Dyslipidemias/drug therapy* ; Flavanones/pharmacology* ; Gene Expression ; Hyperglycemia/drug therapy* ; Insulin/blood ; Lipid Metabolism/drug effects ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Transgenic ; PPAR alpha/metabolism* ; PPAR gamma/metabolism
Keywords
Hyperglycemia ; Hyperlipidemia ; Insulin ; PPAR ; Type 2 diabetes ; Wogonin
Abstract
BACKGROUND & AIMS: Wogonin is a flavonoid extracted from the root of Scutellaria baicalensis Gerogi. We evaluated the therapeutic effects of wogonin using db/db mice. METHODS: Mice received wogonin or vehicle by oral gavage for 2 weeks. Blood glucose, insulin, and cholesterol levels were measured, and liver morphology was observed with histopathological analysis. The mRNA expression levels of PPARα, PPARγ, and adiponectin in the liver and white adipose tissue (WAT) were determined by real-time PCR. Immunoblotting for AMPK and PPARγ, and adipocyte differentiation were investigated in vitro using 3T3-L1 cells. A luciferase assay was used to measure PPARα and PPARγ binding activity. RESULTS: The wogonin group showed decreased weight gain without a change in food intake and improved glucose tolerance. Serum insulin and cholesterol levels in the wogonin group were significantly decreased compared to those in the control group. The wogonin group also showed less accumulation of lipid droplets and glycogen in the liver. PPARα and PPARγ expression levels in the liver and WAT and adiponectin expression level in WAT in the wogonin group were higher than those in the control group. In 3T3-L1 cells, wogonin was shown to stimulate AMPK activation in a dose-dependent manner. The presence of wogonin did not affect adipocyte differentiation or PPARγ protein level during adipogenesis. Notably, wogonin enhanced PPARα but not PPARγ transactivation. CONCLUSIONS: These indicate that wogonin may have beneficial effects on glucose and lipid metabolism related to enhanced PPARα and adiponectin expression via AMPK activation. Importantly, wogonin did not cause deleterious effects, such as weight gain and fatty liver. Wogonin might be a useful therapeutic agent to treat type 2 diabetes
Full Text
http://www.sciencedirect.com/science/article/pii/S0261561413000915
DOI
10.1016/j.clnu.2013.03.013
Appears in Collections:
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Ji Hye(김지혜)
Kim, Jin Moon(김진문)
Bak, Eun-Jung(박은정) ORCID logo https://orcid.org/0000-0002-7976-8594
Yoo, Yun Jung(유윤정) ORCID logo https://orcid.org/0000-0002-0045-9597
Lee, Dong Eun(이동은)
Cha, Jung Heon(차정헌) ORCID logo https://orcid.org/0000-0002-9385-2653
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/98001
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