1 329

Cited 11 times in

Cytotoxicity evaluation of zinc oxide-eugenol and non-eugenol cements using different fibroblast cell lines

 Jae-Sung Kwon  ;  Rasika P. Illeperuma  ;  Jin Kim  ;  Kwang-Mahn Kim  ;  Kyoung-Nam Kim 
 Acta Odontologica Scandinavica, Vol.72(1) : 64-70, 2014 
Journal Title
 Acta Odontologica Scandinavica 
Issue Date
Animals ; Cell Line ; Cell Proliferation/drug effects ; Cell Survival/drug effects* ; Dental Cements* ; Eugenol/pharmacology* ; Fibroblasts/drug effects ; Humans ; Mice ; Zinc Oxide/pharmacology*
biocompatibility ; cytotoxicity ; temporary cements ; fibroblasts
Objectives: Despite being commonly used as temporary cements in dentistry, there is a lack of studies regarding the cytotoxicity of zinc oxide-eugenol (ZOE) and zinc oxide non-eugenol (ZONE) cements. In addition, cytotoxicity evaluation of the materials often involves animal-based cells. Therefore, in this study, a cytotoxicity evaluation of commercially available ZOE and ZONE cements was carried out using both animal and human-based cells. Materials and methods. The extraction or dilution of the extraction from four commercially available cements (two zinc oxide-eugenol and two zinc oxide non-eugenol) was tested for cytotoxicity, using three different cells and a water-soluble treatzolium salt assay. The results were confirmed using a confocal laser microscope following calcein AM and ethidium homodimer-1 staining. Results. The results showed that there was a significant difference in cell viability depending on which cell was used, even when the same material was tested. Generally, L929 showed relatively low cell viability with a low EC50 (effective concentration of extracts that caused 50% of cell viability compared to the control) value compared to both HGF-1 and hTERT-hNOF. Such results were also confirmed by a confocal laser microscope. Conclusions. Careful consideration on interpreting the results for cytotoxicity evaluation of ZOE and ZONE cements is needed when different cells are used.
Full Text
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Jae-Sung(권재성) ORCID logo https://orcid.org/0000-0001-9803-7730
Kim, Kyoung Nam(김경남)
Kim, Kwang Mahn(김광만) ORCID logo https://orcid.org/0000-0002-5235-0294
Kim, Jin(김진)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.