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Cytotoxicity evaluation of zinc oxide-eugenol and non-eugenol cements using different fibroblast cell lines

Authors
 Jae-Sung Kwon  ;  Rasika P. Illeperuma  ;  Jin Kim  ;  Kwang-Mahn Kim  ;  Kyoung-Nam Kim 
Citation
 Acta Odontologica Scandinavica, Vol.72(1) : 64-70, 2014 
Journal Title
 Acta Odontologica Scandinavica 
ISSN
 0001-6357 
Issue Date
2014
Abstract
Objectives: Despite being commonly used as temporary cements in dentistry, there is a lack of studies regarding the cytotoxicity of zinc oxide-eugenol (ZOE) and zinc oxide non-eugenol (ZONE) cements. In addition, cytotoxicity evaluation of the materials often involves animal-based cells. Therefore, in this study, a cytotoxicity evaluation of commercially available ZOE and ZONE cements was carried out using both animal and human-based cells. Materials and methods. The extraction or dilution of the extraction from four commercially available cements (two zinc oxide-eugenol and two zinc oxide non-eugenol) was tested for cytotoxicity, using three different cells and a water-soluble treatzolium salt assay. The results were confirmed using a confocal laser microscope following calcein AM and ethidium homodimer-1 staining. Results. The results showed that there was a significant difference in cell viability depending on which cell was used, even when the same material was tested. Generally, L929 showed relatively low cell viability with a low EC50 (effective concentration of extracts that caused 50% of cell viability compared to the control) value compared to both HGF-1 and hTERT-hNOF. Such results were also confirmed by a confocal laser microscope. Conclusions. Careful consideration on interpreting the results for cytotoxicity evaluation of ZOE and ZONE cements is needed when different cells are used.
Full Text
http://informahealthcare.com/doi/abs/10.3109/00016357.2013.798871
DOI
10.3109/00016357.2013.798871
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
Yonsei Authors
권재성(Kwon, Jae-Sung) ORCID logo https://orcid.org/0000-0001-9803-7730
김경남(Kim, Kyoung Nam)
김광만(Kim, Kwang Mahn)
김진(Kim, Jin)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/97993
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