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Metabolic phenotypes in primary unknown metastatic carcinoma

Authors
 Hye Min Kim  ;  Do Hee Kim  ;  Woo Hee Jung  ;  Ja Seung Koo 
Citation
 Journal of Translational Medicine, Vol.12(2) : 1-13, 2014 
Journal Title
 Journal of Translational Medicine 
ISSN
 1479-5876 
Issue Date
2014
MeSH
Female ; Glucose Transporter Type 1/metabolism ; Glycolysis ; Humans ; Immunohistochemistry ; Male ; Middle Aged ; Mitochondrial Proteins/metabolism ; Neoplasm Metastasis/pathology* ; Neoplasm Proteins/metabolism ; Neoplasms, Unknown Primary/classification ; Neoplasms, Unknown Primary/metabolism* ; Neoplasms, Unknown Primary/pathology* ; Phenotype ; Prognosis ; Stromal Cells/metabolism ; Stromal Cells/pathology ; Succinate Dehydrogenase/metabolism ; Survival Analysis
Keywords
Carcinoma ; Primary unknown ; Metabolism
Abstract
BACKGROUND: The purpose of this study is to evaluate expression of metabolism-related proteins in primary unknown metastatic carcinoma (PUMC) and associated implications for treatment. METHODS: A tissue microarray containing 77 cases of PUMC was constructed and immunohistochemical staining was used to evaluate expression of the following proteins: Glycolysis-related: Glut-1, carbonic anhydrase (CA) IX, and monocarboxylate transporter (MCT) 4; Glutaminolysis-related: glutaminase1 (GLS1), glutamate dehydrogenase (GDH), and amino acid transporter-2 (ASCT2); and Mitochondrial-related: ATP synthase, succinate dehydrogenase (SDH)A, and SDHB. The association between immunohistochemical staining results and clinicopathologic parameters was evaluated. RESULTS: The expression of metabolism-related proteins was different depending on the histologic subtype. Compared to other subtypes, squamous cell carcinomas (SQ) expressed more Glut-1 (p = 0.028), while adenocarcinomas (AD) expressed more SDHB in the stroma (p = 0.025). The expression of metabolism-related proteins was also different depending on the clinical subtypes. Glut-1 was expressed most in the nodal type and the least in carcinomatosis type, when compared to other subtypes (p = 0.021). The metabolic phenotypes also showed other trends: when the stroma showed no glutaminolysis, the tumor mostly invaded lymph node, bone, and brain, while the tumor invaded regions other than lymph node, bone, and brain when the stroma showed glutaminolysis (p = 0.003). When the stroma showed the mitochondrial metabolic type, the histologic subtype was mainly AD, but the non-mitochondrial type was associated more with SQ (P = 0.049). CONCLUSION: For PUMC, the expression of metabolism-related proteins, such as Glut-1 and SDHB, differs in the tumor or stroma depending on the clinical and histologic tumor subtype.
Full Text
http://www.translational-medicine.com/content/12/1/2
DOI
10.1186/1479-5876-12-2
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Koo, Ja Seung(구자승) ORCID logo https://orcid.org/0000-0003-4546-4709
Kim, Hye Min(김혜민) ORCID logo https://orcid.org/0000-0002-2899-9480
Jung, Woo Hee(정우희)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/97970
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