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혈중 싸이클로스포린 농도 측정에 있어 MEIA법과 비교한 ACMIA법의 유용성

Other Titles
 The Efficacy of the Affinity Column-mediated Immunoassay Method in Cyclosporine Concentration Assay Compared with the Microparticle Enzyme Immunoassay Method 
 김석모  ;  주만기  ;  김유선  ;  김순일  ;  김명수  ;  장혜경  ;  최상미  ;  김혜진 
 Journal of the Korean Society for Transplantation, Vol.21(2) : 223-227, 2007 
Journal Title
Journal of the Korean Society for Transplantation(대한이식학회지)
Issue Date
Purpose: Cyclosporine is a potent immunosuppressive drug used in organ transplantation. Because of its substantial toxic effect, narrow therapeutic index, and the inter-individual variability of pharmacokinetics, therapeutic drug monitoring of the whole blood cyclosporine concentration has been recommended. We have investigated the comparability of the results of two immunoassay systems, the affinity column- mediated immunoassay (ACMIA) and the microparticle enzyme immunoassay (MEIA), and compared the differences in the cyclosporine concentrations between the two methods in relation to the hematologic and biochemical values. Methods: One hundred twenty-one blood samples from kidney recipients were used. We used Dimension(R) RxL HM with a CSA Flex(R) reagent cartilage for the ACMIA method and AXSYM(R) Cyclosporine for the MEIA method. Results: Cyclosporine concentrations measured by the ACMIA method (n=121) were closely correlated with those measured by the MEIA method (r=0.948, P<0.0001). The Bland-Altman plot using concentration differences between the two methods and the average of the two methods also showed no specific trends (R2=0.02, P=0.125). Except hematocrit, other hematologic and biochemical value (albumin, total bilirubin) didn``t affect to both cyclosporine level by MEIA and ACMIC method. Both cyclosporine levels determined by the MEIA method and the ACMIA method were positively correlated with hematocrit (P<0.05). Conclusion: The ACMIA method used for a cyclosporine assay is precise and has advantages, including the lack of a required pretreatment procedure, and shows same influence by hematocrit compared with the MEIA.
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1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Myoung Soo(김명수) ORCID logo https://orcid.org/0000-0002-8975-8381
Kim, Soon Il(김순일) ORCID logo https://orcid.org/0000-0002-0783-7538
Kim, Yu Seun(김유선) ORCID logo https://orcid.org/0000-0002-5105-1567
Chang, Hye Kyung(장혜경)
Joo, Man Ki(주만기) ORCID logo https://orcid.org/0000-0002-4112-7003
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