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Tissue transglutaminase is essential for integrin-mediated survival of bone marrow-derived mesenchymal stem cells

DC FieldValueLanguage
dc.contributor.author정남식-
dc.contributor.author황기철-
dc.contributor.author박성하-
dc.contributor.author서혜선-
dc.contributor.author심지영-
dc.contributor.author유경종-
dc.contributor.author임소연-
dc.contributor.author장양수-
dc.contributor.author장우철-
dc.date.accessioned2014-12-21T17:19:05Z-
dc.date.available2014-12-21T17:19:05Z-
dc.date.issued2007-
dc.identifier.issn1066-5099-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/97362-
dc.description.abstractAutologous mesenchymal stem cell (MSC) transplantation therapy for repair of myocardial injury has inherent limitations due to the poor viability of the stem cells after cell transplantation. Adhesion is a prerequisite for cell survival and also a key factor for the differentiation of MSCs. As a novel prosurvival modification strategy, we genetically engineered MSCs to overexpress tissue transglutaminase (tTG), with intention to enhance adhesion and ultimately cell survival after implantation. tTG-transfected MSCs (tTG-MSCs) showed a 2.7-fold and greater than a twofold increase of tTG expression and surface tTG activity, respectively, leading to a 20% increased adhesion of MSCs on fibronectin (Fn). Spreading and migration of tTG-MSCs were increased 4.75% and 2.52%, respectively. Adhesion of tTG-MSCs on cardiogel, a cardiac fibroblast-derived three-dimensional matrix, showed a 33.1% increase. Downregulation of tTG by transfection of small interfering RNA specific to the tTG resulted in markedly decreased adhesion and spread of MSCs on Fn or cardiogel. tTG-MSCs on Fn significantly increased phosphorylation of focal adhesion related kinases FAK, Src, and PI3K. tTG-MSCs showed significant retention in infarcted myocardium by forming a focal adhesion complex and developed into cardiac myocyte-like cells by the expression of cardiac-specific proteins. Transplantation of 1 × 106 MSCs transduced with tTG into the ischemic rat myocardium restored normalized systolic and diastolic cardiac function. tTG-MSCs further restored cardiac function of infarcted myocardium as compared with MSC transplantation alone. These findings suggested that tTG may play an important role in integrin-mediated adhesion of MSCs in implanted tissues.-
dc.description.statementOfResponsibilityopen-
dc.format.extent1431~1438-
dc.relation.isPartOfSTEM CELLS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleTissue transglutaminase is essential for integrin-mediated survival of bone marrow-derived mesenchymal stem cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Internal Medicine (내과학)-
dc.contributor.googleauthorHeesang Song-
dc.contributor.googleauthorWoochul Chang-
dc.contributor.googleauthorKi-Chul Hwang-
dc.contributor.googleauthorNamsik Chung-
dc.contributor.googleauthorYangsoo Jang-
dc.contributor.googleauthorHakbae Lee-
dc.contributor.googleauthorByoung-Hyun Min-
dc.contributor.googleauthorByung-Soo Kim-
dc.contributor.googleauthorKyung-Jong Yoo-
dc.contributor.googleauthorSungha Park-
dc.contributor.googleauthorChi Young Shim-
dc.contributor.googleauthorHye-Sun Seo-
dc.contributor.googleauthorSoyeon Lim-
dc.identifier.doi10.1634/stemcells.2006-0467-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03585-
dc.contributor.localIdA04456-
dc.contributor.localIdA01512-
dc.contributor.localIdA01923-
dc.contributor.localIdA02213-
dc.contributor.localIdA02453-
dc.contributor.localIdA03448-
dc.contributor.localIdA03452-
dc.contributor.localIdA03373-1-
dc.relation.journalcodeJ02683-
dc.identifier.eissn1549-4918-
dc.contributor.alternativeNameChung, Nam Sik-
dc.contributor.alternativeNameHwang, Ki Chul-
dc.contributor.alternativeNamePark, Sung Ha-
dc.contributor.alternativeNameSeo, Hye Sun-
dc.contributor.alternativeNameShim, Chi Young-
dc.contributor.alternativeNameYoo, Kyung Jong-
dc.contributor.alternativeNameLim, So Yeon-
dc.contributor.alternativeNameJang, Yang Soo-
dc.contributor.alternativeNameChang, Woo Chul-
dc.contributor.affiliatedAuthorChung, Nam Sik-
dc.contributor.affiliatedAuthorHwang, Ki Chul-
dc.contributor.affiliatedAuthorPark, Sung Ha-
dc.contributor.affiliatedAuthorSeo, Hye Sun-
dc.contributor.affiliatedAuthorShim, Chi Young-
dc.contributor.affiliatedAuthorYoo, Kyung Jong-
dc.contributor.affiliatedAuthorJang, Yang Soo-
dc.contributor.affiliatedAuthorChang, Woo Chul-
dc.contributor.affiliatedAuthorLim, So Yeon-
dc.rights.accessRightsfree-
dc.citation.volume25-
dc.citation.number6-
dc.citation.startPage1431-
dc.citation.endPage1438-
dc.identifier.bibliographicCitationSTEM CELLS, Vol.25(6) : 1431-1438, 2007-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Thoracic and Cardiovascular Surgery (흉부외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers

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