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Clustering of peptidoglycan recognition protein-SA is required for sensing lysine-type peptidoglycan in insects

Authors
 Ji-Won Park  ;  Chan-Hee Kim  ;  Bok-Luel Lee  ;  Nam-Chul Ha  ;  Won-Jae Lee  ;  Byung-Ha Oh  ;  Jae-Hong Lim  ;  Ji-Hwan Ryu  ;  Hyeon-Hwa Lee  ;  Su-Jin Kim  ;  Kyung-Baeg Roh  ;  Byung-Rok Je  ;  Jung-Hyun Kim 
Citation
 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol.104(16) : 6602-6607, 2007 
Journal Title
 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 
ISSN
 0027-8424 
Issue Date
2007
Abstract
Recognition of lysine-type peptidoglycan by peptidoglycan recognition protein (PGRP)-SA provokes the activation of the Toll and prophenoloxidase pathways. Here we reveal that a soluble fragment of lysine-type peptidoglycan, a long glycan chain with short stem peptides, is a potent activator of the Drosophila Toll pathway and the prophenoloxidase activation cascade in the beetle Tenebrio molitor. Using this peptidoglycan fragment, we present biochemical evidence that clustering of PGRP-SA molecules on the peptidoglycan is required for the activation of the prophenoloxidase cascade. We subsequently highlight that the lysozyme-mediated partial digestion of highly cross-linked lysine-type peptidoglycan dramatically increases the binding of PGRP-SA, presumably by inducing clustering of PGRP-SA, which then recruits the Gram-negative bacteria-binding protein 1 homologue and a modular serine protease containing low-density lipoprotein and complement control protein domains. The crucial role of lysozyme in the prophenoloxidase activation cascade is further confirmed in vivo by using a lysozyme inhibitor. Taken together, we propose a model whereby lysozyme presents a processed form of lysine-type peptidoglycan for clustering of PGRP-SA that recruits Gram-negative bacteria-binding protein 1 and the modular serine protease, which leads to the activation of both the Toll and prophenoloxidase pathways.
Files in This Item:
T200705149.pdf Download
DOI
10.1073/pnas.0610924104
Appears in Collections:
5. Research Institutes (연구소) > Research Center for Human Natural Defense System (생체방어연구센터) > 1. Journal Papers
Yonsei Authors
Ryu, Ji Hwan(유지환)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/97320
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