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Involvement of β2-integrin in ROS-mediated neutrophil apoptosis induced by Entamoeba histolytica

Authors
 Seobo Sim  ;  Soon-Jung Park  ;  Myeong Heon Shin  ;  Kyung-Il Im  ;  Tai-Soon Yong 
Citation
 MICROBES AND INFECTION, Vol.9(11) : 1368-1375, 2007 
Journal Title
 MICROBES AND INFECTION 
ISSN
 1286-4579 
Issue Date
2007
Abstract
Cellular adhesion through β2-integrin (CD18) is an important step in signal transduction leading to apoptosis of human neutrophils, and NADPH oxidase-derived reactive oxygen species (ROS) are essential for neutrophil apoptosis induced by Entamoeba histolytica. Therefore, we investigated the role of β2-integrin-mediated signals in ROS-dependent neutrophil apoptosis induced by E. histolytica. Entamoeba-induced apoptosis was inhibited by pre-incubation of cells with mAb to CD18, but not CD29, suggesting that β2-integrin plays an important role in this response. Moreover, Entamoeba-induced ROS generation in neutrophils was inhibited by mAbs against CD18 or CD11b, but not by mAbs against CD11a, CD11c, or CD29. A combination of d-galactose plus anti-CD18 mAb had a larger inhibitory effect than d-galactose alone on Entamoeba-induced apoptosis and ROS generation. Furthermore, Entamoeba-induced apoptosis and ROS generation were inhibited by pre-treatment of cells with an inhibitor of phosphatidylinositol-3-kinase (PI-3-kinase). These results indicate that β2-integrin and PI-3-kinase are crucial signaling molecules in ROS-dependent apoptosis of neutrophils induced by E. histolytica.
Full Text
http://www.sciencedirect.com/science/article/pii/S1286457907002584
DOI
10.1016/j.micinf.2007.06.013
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Park, Soon Jung(박순정) ORCID logo https://orcid.org/0000-0002-0423-1944
Shin, Myeong Heon(신명헌) ORCID logo https://orcid.org/0000-0001-8207-6110
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Im, Kyung Il(임경일)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/97147
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