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Differential diagnosis of Trichostrongylus and hookworm eggs via PCR using ITS-1 sequence

Authors
 Tai-soon Yong  ;  Jong-ho Lee  ;  Han-jong Rim  ;  Soon-hyung Lee  ;  Woon-mok Sohn  ;  Keeseon S. Eom  ;  Jong-yil Chai  ;  Duk-young Min  ;  Jongweon Lee  ;  Seobo Sim 
Citation
 KOREAN JOURNAL OF PARASITOLOGY, Vol.45(1) : 69-74, 2007 
Journal Title
KOREAN JOURNAL OF PARASITOLOGY
ISSN
 0023-4001 
Issue Date
2007
Abstract
Trichostrongylus eggs observed in cellophane-thick smears are difficult, in practice, to distinguish from hookworm eggs. In order to overcome these limitations, a molecular approach was conducted. A Trichostrongylus colubriformis adult worm was obtained from a human in Laos, which was identified morphologically. ITS-1 sequence of this worm was determined, and found to be most similar with that of T. colubriformis among the Trichostrongylus spp. reported so far. Then, this sequence was compared with those of human hookworm species, Ancylostoma duodenale and Necator americanus, and species-specific oligonucleotide primers were designed. Polymerase chain reaction (PCR) using these primers evidenced specifically amplified PCR products of Trichostrongylus sp., A. duodenale and N. americanus from the eggs of each (520 bp, 690 bp, and 870 bp, respectively). A species-specific PCR technique can be developed in order to study the epidemiology of Trichostrongylus spp. and hookworms in endemic areas.
Files in This Item:
T200701248.pdf Download
DOI
10.3347/kjp.2007.45.1.69
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Sim, Seo Bo(심서보)
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Lee, Jong Weon(이종원)
Lee, Jong Ho(이종호)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/97018
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