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Differential diagnosis of Trichostrongylus and hookworm eggs via PCR using ITS-1 sequence

DC Field Value Language
dc.contributor.author이종원-
dc.contributor.author이종호-
dc.contributor.author심서보-
dc.contributor.author용태순-
dc.date.accessioned2014-12-21T17:08:19Z-
dc.date.available2014-12-21T17:08:19Z-
dc.date.issued2007-
dc.identifier.issn0023-4001-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/97018-
dc.description.abstractTrichostrongylus eggs observed in cellophane-thick smears are difficult, in practice, to distinguish from hookworm eggs. In order to overcome these limitations, a molecular approach was conducted. A Trichostrongylus colubriformis adult worm was obtained from a human in Laos, which was identified morphologically. ITS-1 sequence of this worm was determined, and found to be most similar with that of T. colubriformis among the Trichostrongylus spp. reported so far. Then, this sequence was compared with those of human hookworm species, Ancylostoma duodenale and Necator americanus, and species-specific oligonucleotide primers were designed. Polymerase chain reaction (PCR) using these primers evidenced specifically amplified PCR products of Trichostrongylus sp., A. duodenale and N. americanus from the eggs of each (520 bp, 690 bp, and 870 bp, respectively). A species-specific PCR technique can be developed in order to study the epidemiology of Trichostrongylus spp. and hookworms in endemic areas.-
dc.description.statementOfResponsibilityopen-
dc.format.extent69~74-
dc.relation.isPartOfKOREAN JOURNAL OF PARASITOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleDifferential diagnosis of Trichostrongylus and hookworm eggs via PCR using ITS-1 sequence-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Environmental Medical Biology (환경의생물학)-
dc.contributor.googleauthorTai-soon Yong-
dc.contributor.googleauthorJong-ho Lee-
dc.contributor.googleauthorHan-jong Rim-
dc.contributor.googleauthorSoon-hyung Lee-
dc.contributor.googleauthorWoon-mok Sohn-
dc.contributor.googleauthorKeeseon S. Eom-
dc.contributor.googleauthorJong-yil Chai-
dc.contributor.googleauthorDuk-young Min-
dc.contributor.googleauthorJongweon Lee-
dc.contributor.googleauthorSeobo Sim-
dc.identifier.doi10.3347/kjp.2007.45.1.69-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03144-
dc.contributor.localIdA03153-
dc.contributor.localIdA02193-
dc.contributor.localIdA02424-
dc.relation.journalcodeJ01957-
dc.identifier.eissn1738-0006-
dc.contributor.alternativeNameLee, Jong Weon-
dc.contributor.alternativeNameLee, Jong Ho-
dc.contributor.alternativeNameSim, Seo Bo-
dc.contributor.alternativeNameYong, Tai Soon-
dc.contributor.affiliatedAuthorLee, Jong Weon-
dc.contributor.affiliatedAuthorLee, Jong Ho-
dc.contributor.affiliatedAuthorSim, Seo Bo-
dc.contributor.affiliatedAuthorYong, Tai Soon-
dc.rights.accessRightsfree-
dc.citation.volume45-
dc.citation.number1-
dc.citation.startPage69-
dc.citation.endPage74-
dc.identifier.bibliographicCitationKOREAN JOURNAL OF PARASITOLOGY, Vol.45(1) : 69-74, 2007-
dc.identifier.rimsid53600-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers

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