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정산 산모와 전자간중 산모의 태반에서 Adenosine 수용체의 발현 양상

 김영한  ;  조남훈  ;  박용원  ;  강명화  ;  권자영  ;  황한성 
 Korean Journal of Obstetrics and Gynecology (대한산부인과학회지), Vol.50(5) : 726-734, 2007 
Journal Title
 Korean Journal of Obstetrics and Gynecology (대한산부인과학회지) 
Issue Date
Objective: The aim of the study was to investigate the differential expression of adenosine receptors (ADORs) in the normal and preeclamptic placenta. Methods: Placentas were obtained from women undergoing cesarean section with normal and preeclamptic pregnancies at term. Total RNA was reverse transcribed using ADORs gene specific primers. RT-PCR measurements were made semi-quantitatively. Western blot analysis was performed for protein quantitation. Immunohistochemical staining with anti-adenosine receptor antibodies were employed to localize adenosine receptors in placental tissues. Results: RT-PCR revealed that A2aR, A2bR, and A3R mRNA, not A1 receptor mRNA were expressed in both normal and preeclamptic placenta. Interestingly, there were somewhat higher expressions of A2aR, A2bR, and A3R mRNA in preeclamptic placenta than in normal placenta. Western blotting revealed that A2a, A2b, and A3 receptors were all present in the placental tissue as verified by immunoreactive protein bands. The bands for the A2a, A2b, and A3 receptors were stronger in preeclamptic placenta than in normal placenta. A2a and A2b receptors were detected in endothelial cell, whereas we could not find the staining for A3 receptor in endothelial cells. Importantly, A3 antibody had high intensity of staining in trophoblasts in preeclampsia. Conclusion: To our knowledge, this study is the first to evaluate the expression of ADORs in normal placenta, and to compare ADOR subtypes in normal versus preeclamptic placenta. This study suggests that the specific subtype of ADORs may have a role in the development of preeclampsia.
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Appears in Collections:
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Myung Hwa(강명화)
Kim, Young Han(김영한) ORCID logo https://orcid.org/0000-0003-0645-6028
Park, Yong Won(박용원)
Cho, Nam Hoon(조남훈) ORCID logo https://orcid.org/0000-0002-0045-6441
Hwang, Han Sung(황한성)
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