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프로테오믹스를 이용한 당뇨망막병증 환자의 유리체 분석

Other Titles
 Proteomic Analysis of the Vitreous with Proliferative Diabetic Retinopathy 
 이성진  ;  이성호  ;  권오웅  ;  이성철  ;  박성희 
 Journal of the Korean Ophthalmological Society (대한안과학회지), Vol.48(4) : 573-588, 2007 
Journal Title
 Journal of the Korean Ophthalmological Society (대한안과학회지) 
Issue Date
Diabetic retinopathy ; Electrophoresis ; MALDI-TOF ; Protein ; Vitreous
Purpose: This study analyzed protein alterations between the normal vitreous and the vitreous with proliferative diabetic retinopathy by proteomics to find the proteins which may elicit diabetic retinopathy. Methods: Two-dimensional electrophoresis was used to make the protein map. Image analysis between the spots on each gels by a proteomics based approach were used to reveal vitreous protein alterations which may elicit proliferative diabetic retinopathy. MALDI-TOF/ESI-TOF mass spectrometry also was used to identify altered protein spots on the gel. Results: Of the 110 different spots on each gels, 36 different proteins were identified and among them 23 proteins were altered in the vitreous with proliferative diabetic retinopathy compared with normal vitreous. Nineteen proteins including alpha-1-antitrypsin, Ig G and A, and complement C3 and C4 were increased in the vitreous with proliferative diabetic retinopathy and 4 proteins includng pigment epithelium derived factor were decreased compared to the normal vitreous. Conclusions: The authors found that pigment epithelium derived factor may be the key protein that induces the neovascularization in the vitreous with proliferative diabetic retinopathy. Increased levels of Ig G and A and C3 and C4 is thought to be related to the autoimmune inflammation in early diabetic microangiopathy. Furthermore, proteins such as alpha-1-antitrypsin may contribute to protective functions of the ischemic retinal cells.
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Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Oh Woong(권오웅)
Lee, Sung Chul(이성철) ORCID logo https://orcid.org/0000-0001-9438-2385
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